In the past few years, the extraction of value-added compounds from the anaerobic digestion of glycerol has been an option to add value to this waste because biodiesel production is increasing worldwide. The evolution of research on glycerol valorization by anaerobic digestion has reached the use of high-rate reactors. However, no study has evaluated glycerol digestion in an anaerobic fluidized bed reactor (AFBR), a configuration with potential advantages in methane production. Still, the best operating temperature for high-rate glycerol digestion remains unclear. To clarify these gaps, the present study aimed to compare glycerol digestion in mesophilic AFBR (30 °C) and thermophilic AFBR (55 °C). In both reactors, glycerol concentration was increased from 1.0 to 7.0 g L⁻¹ at a fixed hydraulic retention time of 24 h, resulting in an increase at the organic loading rate from 1.2 to 7.6 kg COD m⁻³ day⁻¹. Thermophilic digestion of glycerol achieved superior removals of organic matter (67.7–94.2%) and methane yield (330.8 mL CH₄ g⁻¹ COD) than the mesophilic digestion (48.6–93.0% and 266.6 mL CH₄ g⁻¹ COD). Additionally, the application of the kinetic model of substrate utilization (modified Stover–Kincannon model) indicated a higher substrate utilization coefficient in the thermophilic AFBR (23.09 g L⁻¹ day⁻¹) than the mesophilic AFBR (7.14 g L⁻¹ day⁻¹). Therefore, the application of glycerol concentrations higher than 7.0 g L⁻¹ in thermophilic AFBR should be further investigated. Also, given only operational results, the application of the AFBR in the two-stage anaerobic digestion of glycerol is recommended.

在过去几年中，从甘油厌氧消化中提取增值化合物已成为为这种废物增加价值的一种选择，因为全球生物柴油产量正在增加。通过厌氧消化使甘油增值的研究进展已达到使用高速反应器的程度。然而，没有研究评估厌氧流化床反应器 (AFBR) 中的甘油消化，这种配置在甲烷生产中具有潜在优势。尽管如此，高速甘油消化的最佳操作温度仍不清楚。为了澄清这些差距，本研究旨在比较中温 AFBR (30 °C) 和嗜热 AFBR (55 °C) 中的甘油消化。在两个反应器中，甘油浓度从 1.0 增加到 7.0 g L ^-1在 24 小时的固定水力停留时间，导致有机负荷率从 1.2 到 7.6 kg COD m ^-3天^{-1 增加}。甘油的嗜热消化实现有机物（67.7-94.2％）和甲烷产率（330.8毫升CH优良清除₄克^-1 COD）低于嗜温消化（48.6-93.0％和266.6毫升CH ₄克^-1 COD）。此外，底物利用动力学模型（改良的 Stover-Kincannon 模型）的应用表明，嗜热 AFBR（23.09 g L ^-1 day ^-1）比中温 AFBR（7.14 g L ^-1 day ^-）具有更高的底物利用系数。¹）。因此，应进一步研究甘油浓度高于 7.0 g L ^-1在嗜热 AFBR 中的应用。此外，仅考虑到操作结果，建议在甘油的两阶段厌氧消化中应用 AFBR。