Developmental & Comparative Immunology ( IF 2.9 ) Pub Date : 2021-06-29 , DOI: 10.1016/j.dci.2021.104186 Hidehiro Kondo 1 , Takumi Fujimura 1 , Fuyuka Murotani 1 , Ryosuke Yazawa 1 , Reoto Tani 1 , Ikuo Hirono 1
Antibodies of cartilaginous fish are of scientific interest due to their phylogenetic position. In the present study, we developed antiserum against IgM of the banded houndshark, Triakis scyllium, and characterized binding activity of the IgM against fish pathogenic bacteria. Pentameric and monomeric IgM antibodies were separated by gel filtration chromatography using high performance liquid chromatography and SDS–PAGE. Antisera were developed by immunizing rabbits with unfractionated IgM antibodies separated by SDS–PAGE electrophoresis. Shark serum antibodies were found to have binding affinity for Aeromonas hydrophila, Vibrio anguillarum, Edwardsiella tarda, and Pseudomonas plecoglossicida antigens but not Lactococcus garvieae by enzyme-linked immunosorbent assay. We speculate the binding activities of shark antibodies may confer protection against certain bacterial pathogens.
中文翻译:
带状猎犬 Triakis scyllium 血清抗体病原体检测活性的初步表征
软骨鱼的抗体由于其系统发育位置而具有科学意义。在本研究中,我们开发了针对带状猎犬Triakis scyllium的 IgM 的抗血清,并表征了 IgM 对鱼类病原菌的结合活性。使用高效液相色谱和 SDS-PAGE 凝胶过滤色谱分离五聚体和单体 IgM 抗体。通过用SDS-PAGE电泳分离的未分级IgM抗体免疫兔来开发抗血清。鲨鱼血清抗体被发现对嗜水气单胞菌、鳗弧菌、迟发爱德华菌和假单胞菌具有结合亲和力。通过酶联免疫吸附测定法检测抗原,而不是加维乳球菌。我们推测鲨鱼抗体的结合活性可能会提供针对某些细菌病原体的保护。