Background The green chemistry approach was developed for the purpose of saving the environment by using green solvents. Applying green analytical chemistry principles to traditional methods is considered a challenge. Acemetacin is a commonly used analgesic prodrug that bioactivates to indomethacin. Objective Developing two simple, eco-friendly chromatographic methods for simultaneous determination of acemetacin and indomethacin. Method The first method is HPLC-DAD. Separation was performed on a Waters XBridge® Shield RP18 (250 × 4.6 mm, 5 µm) analytical column with ethanol–ammonium acetate buffer (50 mM, pH 3.5 ± 0.1; 60:40, v/v) as a mobile phase at a flow rate of 1 mL/min at 25 ± 0.5°C and UV detection at 254 nm. The other method is TLC coupled with densitometric quantification using pre-coated silica TLC plates and butanol–ethyl acetate (70:30, v/v) elution system. The plates were scanned at 254 nm. Results Both methods were validated according to International Conference on Harmonization guidelines. Linearity was confirmed for both over a concentration range of 1–100 µg/mL for the HPLC method and 0.2–7 µg/band for TLC-densitometric method. The methods’ greenness was evaluated by the National Environmental Methods Index, Eco-Scale, Green Analytical Procedure Index metrics and Analytical GREEnness Metric Approach. Conclusions The proposed methods were found to be suitable for determination of studied drugs in their marketed formulations and is suggested for routine analysis in quality control laboratories. Highlights The developed HPLC method shortened the elution time of the analyzed drugs saving more time and money and the TLC method lowered the drugs’ detection limit. HPLC and TLC methods were validated for the assay of acemetacin and indomethacin. The methods’ greenness was evaluated and compared to published methods. The developed HPLC method shortened the elution time of the analyzed drugs, saving time and money and the TLC method lowered the drugs’ detection limit.

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测定阿西美辛和吲哚美辛的环保色谱法；绿色概况评估

背景绿色化学方法是为了通过使用绿色溶剂来保护环境而开发的。将绿色分析化学原理应用于传统方法被认为是一项挑战。阿西美辛是一种常用的镇痛前药，可生物活化为吲哚美辛。目的建立两种简便、环保的色谱方法同时测定阿西美辛和吲哚美辛。方法第一种方法是HPLC-DAD。在 Waters XBridge® Shield RP18 (250 × 4.6 mm, 5 µm) 分析柱上进行分离，使用乙醇-乙酸铵缓冲液 (50 mM, pH 3.5 ± 0.1; 60:40, v/v) 作为流动相流速为 1 mL/min，温度为 25 ± 0.5°C，紫外检测波长为 254 nm。另一种方法是 TLC 与使用预涂硅胶 TLC 板和丁醇-乙酸乙酯（70:30，v/v) 洗脱系统。在 254 nm 处扫描板。结果 两种方法均根据国际协调会议指南进行了验证。HPLC 方法的浓度范围为 1–100 µg/mL，TLC 光密度法的浓度范围为 0.2–7 µg/波段，均证实了线性。方法的绿色性通过国家环境方法指数、生态尺度、绿色分析程序指数指标和分析绿色指标方法进行评估。结论 发现所提出的方法适用于测定其上市制剂中的研究药物，并建议用于质量控制实验室的常规分析。亮点 开发的 HPLC 方法缩短了分析药物的洗脱时间，节省了更多的时间和金钱，TLC 方法降低了药物的检测限。高效液相色谱法和薄层色谱法验证了阿西美辛和吲哚美辛的测定。评估了这些方法的绿色性，并与已发表的方法进行了比较。开发的HPLC方法缩短了分析药物的洗脱时间，节省了时间和金钱，TLC方法降低了药物的检出限。