Abstract

Background

Although yoga is found to be beneficial in the management of type 2 diabetes (T2D), its mechanism of action is poorly understood. T2D is also known to be associated with increased oxidative stress (OS) and DNA damage.

Purpose

This study examines how yoga modulates OS-induced DNA damage and the efficiency of DNA repair in T2D conditions.

Methods

In this assessor-masked randomized clinical trial, T2D subjects (n = 61), aged (Mean ± SD, 50.3 ± 4.2) were randomly allocated into Yoga group (31) that received 10 weeks of yoga intervention and Control (30) with routine exercises. Molecular and biochemical assessments were done before and after the intervention period. Structural Equation Modeling using “R” was used for mediation analysis.

Results

At the end of the 10th week, Yoga group showed significant reduction in DNA damage indicators like Tail Moment (−5.88[95%CI: −10.47 to −1.30]; P = .013) and Olive Tail Moment (−2.93[95%CI: −4.87 to −1.00]; P < .01), oxidative DNA damage marker 8-OHdG (−60.39[95%CI: −92.55 to −28.23]; P < .001) and Fasting Blood Sugar (-22.58[95%CI: −44.33 to −0.83]; P = .042) compared to Control. OGG1 protein expression indicating DNA repair, improved significantly (17.55[95%CI:1.37 to 33.73]; P = .034) whereas Total Antioxidant Capacity did not (5.80[95%CI: -0.86 to 12.47]; P = 0.086). Mediation analysis indicated that improvements in oxidative DNA damage and DNA repair together played a major mediatory role (97.4%) in carrying the effect of yoga.

Conclusion

The beneficial effect of yoga on DNA damage in T2D subjects was found to be mediated by mitigation of oxidative DNA damage and enhancement of DNA repair.

Clinical Trial information

(www.ctri.nic.in) CTRI/2018/07/014825

中文翻译：

---

瑜伽在减轻 2 型糖尿病 DNA 损伤中的作用及其可能机制：一项随机临床试验

摘要

背景

尽管发现瑜伽对治疗 2 型糖尿病 (T2D) 有益，但对其作用机制知之甚少。众所周知，T2D 与氧化应激 (OS) 和 DNA 损伤增加有关。

目的

本研究探讨了瑜伽如何调节 OS 诱导的 DNA 损伤以及 T2D 条件下 DNA 修复的效率。

方法

在这项由评估者掩盖的随机临床试验中，T2D 受试者（n  = 61），年龄（平均值±标准差，50.3±4.2）被随机分配到接受 10 周瑜伽干预的瑜伽组（31）和常规的控制（30）练习。在干预期前后进行分子和生化评估。使用“R”的结构方程建模用于中介分析。

结果

在第 10 周结束时，瑜伽组的 DNA 损伤指标显着降低，例如 Tail Moment (-5.88[95%CI: -10.47 to -1.30]; P  = .013) 和 Olive Tail Moment (-2.93[95%) CI：-4.87 至 -1.00]；P < .01)，氧化性 DNA 损伤标志物 8-OHdG (-60.39[95%CI：-92.55 至 -28.23]；P < .001) 和空腹血糖 (-22.58[ 95%CI：-44.33 至 -0.83]；P  = .042）与对照相比。指示 DNA 修复的 OGG1 蛋白表达显着改善（17.55[95%CI:1.37 至 33.73]；P  = .034）而总抗氧化能力没有改善（5.80[95%CI:-0.86 至 12.47]；P = 0.086)。中介分析表明，氧化性 DNA 损伤和 DNA 修复的改善共同在瑜伽的效果中发挥了主要的中介作用（97.4%）。

结论

瑜伽对 T2D 受试者 DNA 损伤的有益作用被发现是通过减轻氧化性 DNA 损伤和增强 DNA 修复来介导的。

临床试验信息

(www.ctri.nic.in) CTRI/2018/07/014825