Multimodal optical microscopy combines different optical and physiological principles working together to obtain rich perspectives from a single biological event. In this paper, the recent advances in multimodal microscopy systems based on off-axis digital holography and transport-of-intensity equation are reviewed. Both methods used the common-path configuration, and realized simultaneous quantitative phase and fluorescence imaging in 3D space. Feasibility checks with fluorescent micro-beads and live plant cells are presented. The results show that both methods are capable of fast acquisition of the quantitative phase and fluorescence information with digital refocusing ability.

中文翻译：

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多模态显微镜：在 3D 空间中快速获取定量相位和荧光成像

多模态光学显微镜将不同的光学和生理原理结合在一起，从单个生物事件中获得丰富的视角。在本文中，回顾了基于离轴数字全息和强度传输方程的多模态显微镜系统的最新进展。这两种方法都使用了共路径配置，并在 3D 空间中实现了同步定量相位和荧光成像。介绍了荧光微珠和活植物细胞的可行性检查。结果表明，两种方法都能够快速获取具有数字重聚焦能力的定量相位和荧光信息。