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Penicillium chrysogenum-Derived Silver Nanoparticles: Exploration of Their Antibacterial and Biofilm Inhibitory Activity Against the Standard and Pathogenic Acinetobacter baumannii Compared to Tetracycline
Journal of Cluster Science ( IF 2.8 ) Pub Date : 2021-06-28 , DOI: 10.1007/s10876-021-02121-5
Hamed Barabadi , Alireza Mohammadzadeh , Hossein Vahidi , Masoumeh Rashedi , Muthupandian Saravanan , Niloufar Talank , Ahad Alizadeh

This study was aimed to evaluate the antibacterial and biofilm inhibitory activity of Penicillium chrysogenum-derived silver nanoparticles (AgNPs) against the standard and pathogenic Acinetobacter baumannii using a 96-well microtiter plate-based method. The AgNPs were characterized by using UV–Vis, TEM, AFM, XRD, DLS, Zeta potential, and FT-IR. The nanoparticles (NPs) were fabricated with a spherical shape and an average hydrodynamic diameter of 48.2 nm. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AgNPs were found to be 4 and 32 µg/mL respectively, whereas the MIC and MBC of tetracycline were found to be 1024 and 8192 µg/mL against A. baumannii (ATCC 19606). The AgNPs and tetracycline represented considerable biofilm inhibitory activity against both the standard and pathogenic A. baumannii at the studied concentrations. However, the AgNPs depicted higher potency to inhibit the process of biofilm formation of some pathogenic A. baumannii species compared to tetracycline. The AgNPs at the concentration of 0.5*MIC (2 µg/mL) inhibited above 90% biofilm inhibition, whereas tetracycline reached 90% biofilm inhibition at the concentration of 4*MIC (4096 µg/mL) against A. baumannii (ATCC 19606). However, further studies are required to evaluate the biofilm inhibitory efficacy of biogenic AgNPs in vivo.



中文翻译:

产黄青霉衍生的银纳米颗粒:与四环素相比,探索它们对标准和致病鲍曼不动杆菌的抗菌和生物膜抑制活性

本研究旨在使用基于 96 孔微量滴定板的方法评估产黄青霉衍生的银纳米粒子 (AgNPs) 对标准和致病鲍曼不动杆菌的抗菌和生物膜抑制活性。通过使用 UV-Vis、TEM、AFM、XRD、DLS、Zeta 电位和 FT-IR 对 AgNP 进行表征。纳米颗粒 (NPs) 制成球形,平均流体动力学直径为 48.2 nm。发现 AgNPs 的最低抑菌浓度 (MIC) 和最低杀菌浓度 (MBC) 分别为 4 和 32 µg/mL,而四环素对鲍曼不动杆菌的 MIC 和 MBC 分别为 1024 和 8192 µg/mL(ATCC 19606)。AgNPs 和四环素在研究浓度下对标准和致病鲍曼不动杆菌具有相当大的生物膜抑制活性。然而,与四环素相比,AgNPs 具有更高的抑制某些致病性鲍曼不动杆菌生物膜形成过程的效力。浓度为 0.5*MIC (2 µg/mL) 的 AgNPs 抑制了 90% 以上的生物膜,而四环素在 4*MIC (4096 µg/mL) 的浓度下对鲍曼不动杆菌(ATCC 19606) 的生物膜抑制达到了 90% . 然而,还需要进一步的研究来评估生物 AgNPs 在体内的生物膜抑制功效。

更新日期:2021-06-28
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