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Enhancement of immunopotentiation using tetanus toxoid-based nanoparticulate dissolvable microneedles
Biomedical Microdevices ( IF 2.8 ) Pub Date : 2021-06-28 , DOI: 10.1007/s10544-021-00571-2
Mansi Waghchaure 1 , Sharayu Govardhane 1 , Pravin Shende 1
Affiliation  

The main objective of the present study was to prepare and evaluate dissolvable microneedle patch containing nanoparticles of tetanus toxoid without the use of any adjuvant and its immunopotentiation activity. Immunization with microneedles is a novel approach in vaccines delivery with advantages such as convenience, simple, and non-invasive therapy. The gelatin nanoparticles were prepared by a layer-by-layer coating method using polystyrene sulfonate (PSS), polyallylamine hydrochloride (PLA), and PLGA. The filtered gelatin nanoparticles were later dispersed in the aqueous PVP K10 solution and integrated into a mold to develop microneedles. The nanoparticles and their dissolvable microneedle patches were evaluated using particle size, surface charge, entrapment efficiency, SEM analysis, in-vitro, and in-vivo studies. The particle size was found in the order of PLGA-coated nanoparticles > layered gelatin nanoparticles > aminated gelatin nanoparticles > gelatin nanoparticles and aminated gelatin nanoparticles showed maximum entrapment efficiency (92.6 ± 3.25%). The microscopic SEM images showed the spherical-shaped particle formation, verifies that the nanoparticles were formed. The gelatin nanoparticles followed the prolonged release for the period of 8 h whereas the nanoparticle-loaded dissolvable microneedles showed the controlled release pattern for 24 h. Aminated nanoparticulate microneedle showed the highest antibody production against tetanus toxoid. Hence, the nanoparticulate dissolvable microneedles-based immunopotentiation can be used as an alternative for delivery of tetanus toxoid.



中文翻译:

使用基于破伤风类毒素的纳米颗粒可溶解微针增强免疫增强作用

本研究的主要目的是在不使用任何佐剂的情况下制备和评估含有破伤风类毒素纳米颗粒的可溶解微针贴片及其免疫增强活性。微针免疫是一种新型的疫苗递送方法,具有方便、简单和非侵入性治疗等优点。明胶纳米颗粒通过使用聚苯乙烯磺酸盐 (PSS)、聚烯丙胺盐酸盐 (PLA) 和 PLGA 的逐层涂覆方法制备。过滤后的明胶纳米颗粒随后分散在 PVP K10 水溶液中,并集成到模具中以开发微针。使用粒径、表面电荷、包埋效率、SEM 分析、 体外和 体内 研究。发现粒径的顺序为 PLGA 包覆的纳米粒子 > 层状明胶纳米粒子 > 胺化明胶纳米粒子 > 明胶纳米粒子,胺化明胶纳米粒子显示出最大的包埋效率 (92.6 ± 3.25%)。显微 SEM 图像显示球形颗粒形成,证实形成了纳米颗粒。明胶纳米颗粒在 8 小时内持续释放,而负载纳米颗粒的可溶性微针显示控释模式达 24 小时。胺化纳米微粒微针显示出最高的抗破伤风类毒素抗体。因此,基于纳米颗粒可溶解微针的免疫增强可用作递送破伤风类毒素的替代方案。

更新日期:2021-06-28
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