Abstract

The aim of this in vitro study was to evaluate the surface properties of four composite materials with different organic contents and Streptococcus mutans (S. mutans) adhesion to the surfaces after 4 h. TCD-based (CT; Charisma Topaz), Bis-GMA-based (ESQ; Estelite Sigma Quick), UDMA-based (GA;G-aenial Anterior), ormocer-based composites (AF;Admira Fusion) were selected. Polystyrene was used as a control group. Fifteen cylindrical specimens (10 mm diameter × 2 mm depth) were prepared for each group. Surface roughness (SR) was investigated with AFM. Hydrophobicity and surface free energy (SFE) was determined by the sessile drop method. Early adhesion of S. mutans on the materials were evaluated by optical density readouts on a spectrophotometer. Surface morphology and S. mutans bacterial adhesion (SMBA) were examined using SEM. Data were analyzed by ANOVA, post-hoc Dunnett (p < 0.001), and Tukey tests (p < 0.05).Spearman’s rho coefficient was used to investigate the correlation between SR and SMBA. There was a statistically significant difference between the test groups in terms of SR (p < 0.05).AF showed the highest roughness value while ESQ showed the lowest. No statistically significant difference was observed between the test groups in terms of total SFE and hydrophobicity (p > 0.05) while a statistically significant difference was obtained between the test groups in terms of surface SMBA (p < 0.05).The highest SMBA was observed in GA while ESQ showed the lowest. There was a significant correlation between SMBA and SR in the AF (r = 0.929; p = 0.001), CT (r = 0.976; p = 0.001) and ESQ(r = 0.731; p = 0.040).There is a correlation between the SR of composite materials with different organic contents and SMBA. It can be stated that the Bis-GMA based composite material has clinically acceptable physical properties due to its low SR and S. mutans adhesion.

摘要

这项体外研究的目的是评估四种具有不同有机物含量的复合材料的表面性质和变形链球菌 (S. mutans)在 4 小时后对表面的粘附性。选择了基于 TCD（CT；Charisma Topaz）、基于 Bis-GMA（ESQ；Estelite Sigma Quick）、基于 UDMA（GA；G-aenial Anterior）、基于 ormocer 的复合材料（AF；Admira Fusion）。聚苯乙烯用作对照组。每组准备 15 个圆柱形样品（直径 10 mm × 深 2 mm）。用 AFM 研究表面粗糙度 (SR)。疏水性和表面自由能 (SFE) 采用固滴法测定。变形链球菌的早期粘附通过分光光度计上的光密度读数评估材料的质量。使用 SEM 检查表面形态和变形链球菌细菌粘附 (SMBA)。采用方差分析、事后Dunnett ( p <  0.001)和Tukey检验( p <  0.05)对数据进行分析。Spearman的rho系数用于研究SR和SMBA之间的相关性。各测试组的 SR 差异有统计学意义（p <  0.05）。AF 的粗糙度值最高，而 ESQ 的粗糙度值最低。在总 SFE 和疏水性方面，测试组之间没有观察到统计学上的显着差异（p > 0.05)，而测试组之间的表面 SMBA 差异有统计学意义( p <  0.05)。在 GA 中观察到 SMBA 最高，而 ESQ 最低。SMBA 与 SR 在 AF ( r  = 0.929; p  = 0.001)、CT ( r  = 0.976; p  = 0.001) 和 ESQ ( r  = 0.731; p  = 0.040) 之间存在显着相关性。不同有机物含量复合材料的SR和SMBA。可以说，基于 Bis-GMA 的复合材料由于其低 SR 和变形链球菌粘附性而具有临床可接受的物理性能。