SummaryTo explore the effect of lncRNA TINCR on the biological behaviours of trophoblasts, we detected and analyzed the expression of terminal differentiation-induced non-protein coding RNA (TINCR) in the placenta tissues of pre-eclamptic and non-pre-eclamptic pregnant women. The gain- and loss-of-function of TINCR was performed to examine the proliferation, migration and invasion abilities of Htr-8/Svneo cells. The levels of epithelial–mesenchymal transition (EMT)-related proteins, cyclin and Wnt/β-catenin pathway were detected. High expression of lncRNA TINCR appeared in placental tissues of patients with pre-eclampsia. The proliferation, invasion and migration of Htr-8/Svneo cells were promoted by TINCR downregulation; the cells were transited from G0/G1 to S phase; and EMT was promoted and the Wnt/β-catenin pathway was activated. In summary, the downregulation of lncRNA TINCR activated the Wnt/β-catenin pathway and promoted the proliferation, invasion and migration of Htr-8/Svneo cells. This study may provide a theoretical basis for treatment of patients with pre-eclampsia.

中文翻译：

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lncRNA TINCR在先兆子痫患者胎盘中的表达及其对滋养细胞生物学行为的影响

摘要为探讨lncRNA TINCR对滋养细胞生物学行为的影响，我们检测并分析了先兆子痫和非先兆子痫孕妇胎盘组织中终末分化诱导的非蛋白编码RNA（TINCR）的表达。进行 TINCR 功能的获得和丧失以检查 Htr-8/Svneo 细胞的增殖、迁移和侵袭能力。检测上皮-间质转化（EMT）相关蛋白、细胞周期蛋白和Wnt/β-连环蛋白通路的水平。lncRNA TINCR高表达出现在先兆子痫患者的胎盘组织中。TINCR下调促进了Htr-8/Svneo细胞的增殖、侵袭和迁移；细胞从G0/G1期过渡到S期；EMT 被促进，Wnt/β-catenin 通路被激活。总之，lncRNA TINCR的下调激活了Wnt/β-catenin通路，促进了Htr-8/Svneo细胞的增殖、侵袭和迁移。本研究可为先兆子痫患者的治疗提供理论依据。