Since the inception of high throughput single-cell RNASeq approaches in 2015 [1, 2], the field of Genomic Cytometry has been maturing at a rapid pace. As part of this maturation, Genomic Cytometry is no longer restricted to RNA analysis but can be used to allow deep interrogation of RNA [3], DNA [4, 5], protein [6, 7], and epigenetic states [8]. Moreover, the ability to combine these modalities onto a single cell is ushering in a new era of single-cell multiomics [9-11].

The recent paper from Corselli et al. [12] combined a targeted scRNAseq approach with oligo AbSeq to simultaneously follow T-cells during in vitro stimulation. This allowed the authors to track a panel of 399 immune-related transcripts and a panel of 38 cell surface proteins over the course of a 14-day stimulation period. Importantly, results from the multiomic comparison were compared with traditional fluorescence flow cytometry. This helps understand how a multiomics approach compares to traditional gold standard methods. The comparison here would suggest that while genomic cytometry is maturing, it is still in its infancy and requires careful attention to see it mature into a robust reliable method with results identical to the current gold standard.

自 2015 年高通量单细胞 RNASeq 方法问世 [ 1, 2 ] 以来，基因组流式细胞仪领域一直在快速成熟。作为这种成熟的一部分，基因组流式细胞仪不再局限于 RNA 分析，而是可用于对 RNA [ 3 ]、DNA [ 4, 5 ]、蛋白质 [ 6, 7 ] 和表观遗传状态 [ 8 ] 进行深入研究。此外，将这些模式结合到单个细胞上的能力正在开启单细胞多组学的新时代 [ 9-11 ]。

Corselli 等人最近的论文。[ 12 ] 将靶向 scRNAseq 方法与寡核苷酸 AbSeq 相结合，在体外刺激期间同时跟踪 T 细胞。这使作者能够在 14 天的刺激期间跟踪一组 399 种免疫相关转录物和一组 38 种细胞表面蛋白。重要的是，将多组学比较的结果与传统的荧光流式细胞仪进行了比较。这有助于了解多组学方法与传统黄金标准方法的比较。这里的比较表明，虽然基因组细胞术正在成熟，但它仍处于起步阶段，需要仔细注意才能将其成熟为一种稳健可靠的方法，其结果与当前的黄金标准相同。