The asymmetric distribution of lipids on the inner and outer membranes of a cell plays a pivotal role in the physiological and immunological activities of life. It has inspired the elaboration of synthetic asymmetric vesicles for the discovery of advanced materials and functions. The asymmetric vesicles were generally prepared by amphiphilic block copolymers. We herein report on the formation of asymmetric vesicles self-assembled by amphiphilic sequence-controlled polymers with two hydrophilic segments SU and TEO. We also developed an efficient fluorescence titration method with europium(III) ions (Eu³⁺) to determine the uneven distribution of SU and TEO. SU units are preferentially located on the outer membrane and TEO on the inner membrane of the resulting vesicles, which is facilitated by the electrostatic repulsion of SU and the U-shaped folding of the hydrophobic backbone of the resulting polymers. This work shows that sequence-controlled polymers with alternating monomer sequence provide a powerful toolbox for the elaboration of important yet challenging self-assembled structures for emerging functions and properties.

中文翻译：

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由两亲序列控制聚合物自组装的不对称囊泡

脂质在细胞内外膜上的不对称分布在生命的生理和免疫活动中起着举足轻重的作用。它激发了合成不对称囊泡的研究，以发现先进的材料和功能。不对称囊泡通常由两亲嵌段共聚物制备。我们在此报告了由具有两个亲水链段SU和TEO的两亲序列控制聚合物自组装形成的不对称囊泡。^{我们还开发了一种使用铕 (III) 离子 (Eu 3+} )的有效荧光滴定方法来确定SU和TEO的不均匀分布。苏单元优先位于所得囊泡的外膜上，而TEO位于所得囊泡的内膜上，这得益于SU的静电排斥和所得聚合物疏水骨架的 U 形折叠。这项工作表明，具有交替单体序列的序列控制聚合物为研究重要但具有挑战性的自组装结构提供了一个强大的工具箱，以实现新兴的功能和特性。