To investigate soil microbial community dynamics in sediment microbial fuel cells (MFCs), this study applied nonhydric (D) and hydric (S) soils to single-chamber and mediator-free MFCs. Glucose was also used to enrich microorganisms in the soils. The voltage outputs of both the D and S sediment MFCs increased over time but differed from each other. The initial open circuit potentials were 345 and 264 mV for the D and S MFCs. The voltage output reached a maximum of 503 and 604 mV for D and S on days 125 and 131, respectively. The maximum power densities of the D and S MFCs were 2.74 and 2.12 mW m⁻², analyzed on day 50. Clustering results revealed that the two groups did not cluster after glucose supplementation and 126 days of MFC function. The change in Geobacter abundance was consistent with the voltage output, indicating that these bacteria may act as the main exoelectrogens on the anode. Spearman correlation analysis demonstrated that, in the D soils, Geobacter was positively correlated with Dialister and negatively correlated with Bradyrhizobium, Kaistobacter, Pedomicrobium, and Phascolarctobacterium; in the S soils, Geobacter was positively correlated with Shewanella and negatively correlated with Blautia. The results suggested that different soil sources in the MFCs and the addition of glucose as a nutrient produced diverse microbial communities with varying voltage output efficiencies.

为了研究沉积物微生物燃料电池 (MFC) 中的土壤微生物群落动态，本研究将非水合 (D) 和水合 (S) 土壤应用于单室和无介质 MFC。葡萄糖也被用来丰富土壤中的微生物。D 和 S 沉积物 MFC 的电压输出随时间增加，但彼此不同。D 和 S MFC 的初始开路电位为 345 和 264 mV。在第 125 天和第 131 天，D 和 S 的电压输出分别达到最大值 503 和 604 mV。D 和 S MFC 的最大功率密度为 2.74 和 2.12 mW m ^-2，在第 50 天进行分析。聚类结果显示两组在补充葡萄糖和 MFC 功能 126 天后没有聚类。地杆菌的变化丰度与电压输出一致，表明这些细菌可能是阳极上的主要产电原。Spearman 相关分析表明，在 D 土壤中，Geobacter与Dialister呈正相关，与Bradyrhizobium、Kaistobacter、Pedomicrobium和Phascolarctobacterium呈负相关；在 S 土壤中，Geobacter与Shewanella呈正相关，与Blautia 呈负相关。结果表明，MFC 中的不同土壤来源和葡萄糖作为营养物的添加产生了具有不同电压输出效率的不同微生物群落。