Pentraxin 3 (PTX3) is synthesized locally and released into the circulation, reflecting local inflammation in the cardiovascular system. Therefore, we conducted a study to explore the effect of PTX3 in spontaneously hypertensive heart failure (SHHF) rats. Sprague Dawley (SD) and SHHF rats were treated with recombinant PTX3 protein, and the blood pressure (BP) and echocardiographic parameters were collected. Radioimmunoassay, enzyme immunoassay and enzyme-linked immunosorbent assay (ELISA) were applied to detect plasma levels of atrial/B-type natriuretic peptide (ANP/BNP) and PTX3. The pathological changes in the myocardial tissues were observed by hematoxylin and eosin (HE) and Masson stainings. The mRNA and protein expressions were detected by quantitative real-time reverse-transcription polymerase chain reaction (qPCR) and western blotting. Cardiomyocyte apoptosis was evaluated by TUNEL staining and DNA fragmentation test. Increased plasma concentrations of PTX3 were found in SHHF rats compared with SD rats, which was further enhanced by recombinant PTX3 protein. After injection with recombinant PTX3 protein, the heart function was improved in SHHF rats with the decreased systolic and diastolic BP, and the reduced plasma levels of ANP and BNP. Moreover, PTX3 improved the myocardial damage and interstitial fibrosis in SHHF rats with reduced cardiomyocyte apoptosis and decreased mRNA expressions of pro-inflammatory factors in myocardial tissues. PTX3 could decrease the BP and plasma levels of ANP and BNP in SHHF rats, as well as improve the inflammation, cardiomyocyte apoptosis, and pathological changes of myocardial tissues, suggesting it may be a useful intervention in the treatment of SHHF.

Pentraxin 3 (PTX3) 在局部合成并释放到循环中，反映了心血管系统的局部炎症。因此，我们进行了一项研究，以探讨 PTX3 在自发性高血压心力衰竭 (SHHF) 大鼠中的作用。Sprague Dawley（SD）和SHHF大鼠用重组PTX3蛋白治疗，并收集血压（BP）和超声心动图参数。应用放射免疫法、酶免疫法和酶联免疫吸附法（ELISA）检测血浆心房/B型利钠肽（ANP/BNP）和PTX3水平。用苏木精伊红(HE)和Masson染色观察心肌组织的病理变化。通过定量实时逆转录聚合酶链反应（qPCR）和蛋白质印迹检测mRNA和蛋白质的表达。通过TUNEL染色和DNA片段化试验评估心肌细胞凋亡。与 SD 大鼠相比，在 SHHF 大鼠中发现 PTX3 的血浆浓度增加，重组 PTX3 蛋白进一步增强了这种浓度。注射重组PTX3蛋白后，SHHF大鼠心功能得到改善，收缩压和舒张压降低，血浆ANP和BNP水平降低。此外，PTX3改善了SHHF大鼠的心肌损伤和间质纤维化，减少了心肌细胞凋亡，降低了心肌组织中促炎因子的mRNA表达。PTX3可降低SHHF大鼠血压和血浆ANP、BNP水平，改善心肌组织炎症、心肌细胞凋亡和病理改变，