Bulletin of Experimental Biology and Medicine ( IF 0.7 ) Pub Date : 2021-06-26 , DOI: 10.1007/s10517-021-05210-7 N I Gulyaeva 1 , V E Vladimirskii 1 , E V Vladimirskii 1 , G G Freind 1
The effects of hydrogen sulfide baths and sulfide-silt mud applications on the abdominal wall were studied in outbred white rats. Heart preparations stained with hematoxylin and eosin were analyzed and the mean numbers of cells expressing Ki-67, Nkx-2.5, and mesenchymal stem cell markers were determined. Hydrogen sulfide balneotherapy promoted the development of blood capillaries in rat hearts and an increased the expression of CD34. A decrease in the regenerative potential of the myocardium was found due to a decrease in the content of proliferating cells and cells expressing CD73, CD90, and CD105, and also due to the absence of cells stained for cardiomyogenic differentiation marker Nkx-2.5.
中文翻译:
硫化氢浴疗法作用下心脏的形态学和免疫组化特征
在远交白大鼠中研究了硫化氢浴和硫化物淤泥应用对腹壁的影响。分析了用苏木精和伊红染色的心脏制剂,并确定了表达 Ki-67、Nkx-2.5 和间充质干细胞标志物的细胞的平均数量。硫化氢浴疗法促进了大鼠心脏毛细血管的发育并增加了CD34的表达。由于增殖细胞和表达 CD73、CD90 和 CD105 的细胞的含量减少,以及由于心肌分化标志物 Nkx-2.5 染色细胞的缺失,发现心肌的再生潜能降低。