Probiotics are considered a promising alternative to antibiotics for Salmonella disease control. However, probiotics fed to poultry and humans lose most of their activity in the stomach because of their sensitivity to gastric fluids. For this purpose, we developed a universal platform for probiotics, shaped as micrometer-scale conical frustums based on microfabrication technology. The casted probiotics produced through molding/demolding processes were coated with a protection layer made of an anionic copolymer, Eudragit S 100. The samples exhibited a gradual release in a simulated gastric fluid (SGF), followed by a rapid stepwise release upon exposure to a simulated intestine fluid (SIF). The encapsulation, viability, and environmental stability were characterized by using Lactobacillus acidophilus (L. acidophilus) encapsulated probiotic formulations. The microfabricated probiotic patch provided protection to encapsulated L. acidophilus cells against harsh digestive conditions and environmental storage conditions. This study showed that microfabricated probiotic formulations can enhance the efficacy of probiotics and result in a dose-sparing effect, reducing the required amount of cells and costs.

中文翻译：

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用于抑制沙门氏菌的微加工益生菌制剂

益生菌被认为是控制沙门氏菌病的抗生素的有前途的替代品。然而，喂给家禽和人类的益生菌由于对胃液敏感而在胃中失去大部分活性。为此，我们开发了一个通用的益生菌平台，其形状为基于微加工技术的微米级圆锥截头体。通过模塑/脱模工艺生产的浇注益生菌涂有由阴离子共聚物 Eudragit S 100 制成的保护层。 样品在模拟胃液 (SGF) 中逐渐释放，然后在暴露于模拟肠液（SIF）。封装、生存能力和环境稳定性通过使用来表征嗜酸乳杆菌( L. acidophilus ) 封装的益生菌制剂。微型益生菌贴片为封装的嗜酸乳杆菌细胞提供保护，使其免受恶劣的消化条件和环境储存条件的影响。这项研究表明，微加工的益生菌制剂可以提高益生菌的功效并产生剂量节约效应，减少所需的细胞数量和成本。