Abstract

Context

Sheng Xue Fang (SXF) has been used to treat anaemia for decades with good efficacy.

Objective

To study the effect and possible mechanism of SXF to restore haematopoietic function.

Materials and methods

Balb/c mice (10 per/group, half male, half female) were treated with SXF (three dose groups, 8.5, 17, and 22.1 g/kg) by gavage for 14 days, and cyclophosphamide (80 mg/kg) was injected on days 10–12. Only injection of cyclophosphamide (negative control) or physiological saline (blank control) were included as controls. The spleen and femur were processed for histopathology. Active components and the target of SXF were screened. The target was used for gene enrichment and protein-protein interaction (PPI) analysis.

Results

Red blood cell relative changes in the SXF group (low: −5.50 ± 1.58%; medium: −11.11 ± 4.15%; high: −8.81 ± 2.67%) and relative negative control (26.21 ± 2.51%) significantly increased (all p < 0.01) in female mice. Haemoglobin and red blood cell-specific volume showed the same trend. However, SXF did not have significant effects on male mice. Splenic index in the medium group (4.44 ± 0.46%) relative negative control (3.38 ± 0.10%) significantly improved (p < 0.01) in female mice. Using network pharmacology, 77 active components and 337 targets were screened from SXF. These targets are closely related to the mitogen-activated protein kinase pathway.

Conclusions

SXF has good clinical application potential. However, the mechanism requires in-depth research. Our findings are of great significance in anaemia treatment and provide a new perspective for Chinese medicine research.

中文翻译：

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生血方对环磷酰胺致贫血小鼠模型的治疗作用

摘要

语境

生血方 (SXF) 几十年来一直用于治疗贫血，疗效良好。

客观的

研究SXF恢复造血功能的作用及可能机制。

材料和方法

Balb/c 小鼠（每组 10 只，半雄性，半雌性）用 SXF（三个剂量组，8.5、17 和 22.1 g/kg）灌胃 14 天，环磷酰胺（80 mg/kg）在第 10-12 天注射。仅注射环磷酰胺（阴性对照）或生理盐水（空白对照）作为对照。对脾脏和股骨进行组织病理学处理。筛选了活性成分和 SXF 的目标。该靶点用于基因富集和蛋白质-蛋白质相互作用 (PPI) 分析。

结果

SXF 组的红细胞相对变化（低：-5.50 ± 1.58%；中：-11.11 ± 4.15%；高：-8.81 ± 2.67%）和相对阴性对照（26.21 ± 2.51%）显着增加（所有p  < 0.01) 在雌性小鼠中。血红蛋白和红细胞比容呈现相同的趋势。然而，SXF 对雄性小鼠没有显着影响。在雌性小鼠中，中等组的脾指数（4.44 ± 0.46%）相对阴性对照（3.38 ± 0.10%）显着改善（p  < 0.01）。使用网络药理学，从 SXF 中筛选出 77 个活性成分和 337 个靶点。这些靶点与丝裂原活化蛋白激酶途径密切相关。

结论

SXF具有良好的临床应用潜力。然而，该机制需要深入研究。我们的研究结果对贫血的治疗具有重要意义，为中医药研究提供了新的视角。