Abstract

In nuclear and mitochondrial DNA, 8-hydroxy-2′-deoxyguanosine (8-OHdG) is one of the predominant forms of reactive oxygen species (ROSs) lesions, which commonly used as a biomarker for oxidative stress. Studies showed that the different nanomaterials can induce toxicity by ROSs in human body. So, this study is going to review the studies about oxidative DNA damage caused by occupational exposure to nanomaterials, using 8-OHdG biomarker.

Systematic review was managed based on Cochrane systematic review guideline. Literature search was conducted in scientific databases with the main terms of “biomarkers,” “biological markers,” combined with “occupational exposure” and “nanomaterials.” All papers in the field of occupational exposure to nanomaterials until 2020 December were included. To evaluate the quality and bias of studies, GRADE method (Grading of Recommendations, Assessment, Development, and Evaluation) was used.

Two hundred twenty-six studies were primarily achieved. By considering the inclusion criteria, overall 8 articles were selected. The majority of the studies were classified as the moderate quality studies (six studies). Also, the study-level bias was critical. This review shows that there is a significant relationship between job title and amount of produced nanomaterials and the existence of 8-OHdG. Also, the levels of 8-OHdG can be measured in urine, blood, and inhalation samples by instrumental procedures.

Oxidative damages are an important threat for workers exposed to nanomaterial. Blood and EBC 8-OHdG level can be introduced as a biomarker for metal nanomaterials, but urinary 8-OHdG needs to be taken with caution. So, it is recommended that evaluation not be solely based on one biomarker.

摘要

核DNA和线粒体DNA，8-羟基-2 '脱氧鸟苷（8-OHdG水平）是其通常用作氧化应激的生物标记物的活性氧（ROSS）病变，的主要形式之一。研究表明，不同的纳米材料可以通过活性氧在人体内诱导毒性。因此，本研究将使用 8-OHdG 生物标志物来回顾有关职业暴露于纳米材料引起的 DNA 氧化损伤的研究。

系统评价根据 Cochrane 系统评价指南进行管理。在科学数据库中进行文献检索，主要术语为“生物标志物”、“生物标志物”，并结合“职业暴露”和“纳米材料”。包括截至 2020 年 12 月的纳米材料职业暴露领域的所有论文。为了评估研究的质量和偏倚，使用了 GRADE 方法（推荐分级、评估、开发和评估）。

初步完成了 226 项研究。综合考虑纳入标准，共选出8篇文章。大多数研究被归类为中等质量研究（六项研究）。此外，研究层面的偏见也很关键。该综述表明，职位名称和纳米材料的产量与 8-OHdG 的存在之间存在显着关系。此外，可以通过仪器程序测量尿液、血液和吸入样品中的 8-OHdG 水平。

氧化损伤对接触纳米材料的工人来说是一个重要的威胁。可以引入血液和 EBC 8-OHdG 水平作为金属纳米材料的生物标志物，但需要谨慎对待尿液 8-OHdG。因此，建议不要仅基于一种生物标志物进行评估。