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Cholinergic boutons are closely associated with excitatory cells and four subtypes of inhibitory cells in the inferior colliculus
Journal of Chemical Neuroanatomy ( IF 2.8 ) Pub Date : 2021-06-26 , DOI: 10.1016/j.jchemneu.2021.101998
Nichole L Beebe 1 , Brett R Schofield 1
Affiliation  

Acetylcholine (ACh) is a neuromodulator that has been implicated in multiple roles across the brain, including the central auditory system, where it sets neuronal excitability and gain and affects plasticity. In the cerebral cortex, subtypes of GABAergic interneurons are modulated by ACh in a subtype-specific manner. Subtypes of GABAergic neurons have also begun to be described in the inferior colliculus (IC), a midbrain hub of the auditory system. Here, we used male and female mice (Mus musculus) that express fluorescent protein in cholinergic cells, axons, and boutons to look at the association between ACh and four subtypes of GABAergic IC cells that differ in their associations with extracellular markers, their soma sizes, and their distribution within the IC. We found that most IC cells, including excitatory and inhibitory cells, have cholinergic boutons closely associated with their somas and proximal dendrites. We also found that similar proportions of each of four subtypes of GABAergic cells are closely associated with cholinergic boutons. Whether the different types of GABAergic cells in the IC are differentially regulated remains unclear, as the response of cells to ACh is dependent on which types of ACh receptors are present. Additionally, this study confirms the presence of these four subtypes of GABAergic cells in the mouse IC, as they had previously been identified only in guinea pigs. These results suggest that cholinergic projections to the IC modulate auditory processing via direct effects on a multitude of inhibitory circuits.



中文翻译:

胆碱能小结与下丘中的兴奋性细胞和四种抑制性细胞亚型密切相关

乙酰胆碱 (ACh) 是一种神经调节剂,涉及整个大脑的多种作用,包括中枢听觉系统,它设置神经元的兴奋性和增益并影响可塑性。在大脑皮层中,GABA 能中间神经元的亚型受 ACh 以亚型特异性方式调节。GABAergic 神经元的亚型也开始在下丘 (IC) 中被描述,下丘 (IC) 是听觉系统的中脑枢纽。在这里,我们使用雄性和雌性小鼠(Mus musculus) 在胆碱能细胞、轴突和 boutons 中表达荧光蛋白,以观察 ACh 与 GABAergic IC 细胞的四种亚型之间的关联,这些亚型与细胞外标记物的关联、它们的体细胞大小以及它们在 IC 内的分布不同。我们发现大多数 IC 细胞,包括兴奋性和抑制性细胞,都具有与其胞体和近端树突密切相关的胆碱能 boutons。我们还发现,四种 GABA 能细胞亚型中每一种的相似比例都与胆碱能细胞密切相关。IC 中不同类型的 GABA 能细胞是否受到差异调节仍不清楚,因为细胞对 ACh 的反应取决于存在的 ACh 受体类型。此外,这项研究证实了小鼠 IC 中存在这四种 GABA 能细胞亚型,因为它们以前只在豚鼠中被发现。这些结果表明,对 IC 的胆碱能投射通过对大量抑制电路的直接影响来调节听觉处理。

更新日期:2021-07-01
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