The popularity of free-range and organic poultry has increased in recent years due to consumer demands. A study was conducted to evaluate how the presence of organic dairy cattle manure could influence the environmental and egg microbiology of organic free-range laying flocks. A flock of brown egg layers was split and maintained in a rotational paddock grazing schedule of which was free of or exposed to organic dairy manure. Environmental and egg sampling occurred approximately every 8 wk between 20 and 44 wk of age for a presence of Listeria spp., Campylobacter spp., Salmonella spp., and enumeration of Enterobacteriaceae. There was no significant difference in the occurrence of Listeria spp., Campylobacter spp. and Salmonella spp. in environmental and egg samples between integrated and control grazing organic free-range flocks. A random sampling of Enterobacteriaceae colonies produced 211 viable isolates for biochemical identification. Seventeen genera, species, or serotypes were identified. There was a greater (P < 0.05) prevalence of total coliforms found on shell emulsion, egg contents, nest box straw, and forage samples from integrated organic free-range compared to control organic free-range flocks. Seasons of the year impacted microbial levels recovered from environmental and egg samples, with which summer having the highest level of all populations monitored. Additional studies are needed to fully understand the effect of mixed production rotational grazing on the prevalence of pathogens and Enterobacteriaceae on organic nest-run eggs and the grazing environment.

近年来，由于消费者的需求，散养和有机家禽越来越受欢迎。进行了一项研究，以评估有机奶牛粪便的存在如何影响有机自由放养产蛋群的环境和鸡蛋微生物学。一群棕色鸡蛋被分开并保持在轮换围场放牧计划中，该计划没有或暴露于有机乳制品粪便。对于李斯特菌属、弯曲杆菌属、沙门氏菌属和肠杆菌科的存在，在 20 到 44 周龄之间大约每 8 周进行一次环境和鸡蛋采样。李斯特菌发生率无显着差异.，弯曲杆菌属。和沙门氏菌。在综合和控制放牧有机自由放养鸡群之间的环境和鸡蛋样品中。肠杆菌科菌落的随机抽样产生了 211 个用于生化鉴定的可行分离株。鉴定了 17 个属、种或血清型。有一个更大的（P< 0.05) 与对照有机自由放养鸡群相比，在壳乳剂、鸡蛋内容物、巢箱秸秆和来自综合有机自由放养鸡群的草料样品中发现的总大肠菌群的流行率。一年中的季节影响了从环境和鸡蛋样本中回收的微生物水平，其中夏季是所有受监测种群中水平最高的。需要更多的研究来充分了解混合生产轮牧对病原体和肠杆菌对有机巢跑蛋和放牧环境的流行的影响。