Microscale fermentation systems are important high throughput tools in clone selection, and bioprocess set up and optimization, since they provide several parallel experiments in controlled conditions of pH, temperature, agitation, and gas flow rate. In this work we evaluated the performance of biotechnologically relevant strains with different respiratory requirements in the micro-Matrix microbioreactor. In particular Escherichia coli K4 requires well aerated fermentation conditions to improve its native production of chondroitin-like capsular polysaccharide, a biomedically attractive polymer. Results from batch and fed-batch experiments demonstrated high reproducibility with those obtained on 2 L reactors, although highlighting a pronounced volume loss for longer-term experiments. Basfia succiniciproducens and Actinobacillus succinogenes need CO₂ addition for the production of succinic acid, a building block with several industrial applications. Different CO₂ supply modes were tested for the two strains in 24 h batch experiments and results well compared with those obtained on lab-scale bioreactors. Overall, it was demonstrated that the micro-Matrix is a useful scale-down tool that is suitable for growing metabolically different strains in simple batch process, however, a series of issues should still be addressed in order to fully exploit its potential.

中文翻译：

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不同工业相关微生物菌株在好氧和厌氧条件下的微生物反应器（微基质）潜力

微型发酵系统是克隆选择、生物过程设置和优化中重要的高通量工具，因为它们在 pH、温度、搅拌和气体流速的受控条件下提供了多个平行实验。在这项工作中，我们评估了具有不同呼吸要求的生物技术相关菌株在微基质微生物反应器中的性能。特别是大肠杆菌K4 需要良好通风的发酵条件，以提高其天然产生的软骨素样荚膜多糖，一种具有生物医学吸引力的聚合物。分批和分批补料实验的结果表明，与在 2 L 反应器上获得的结果具有较高的重现性，尽管突出了长期实验的明显体积损失。Basfia succiniciproducens和Actinobacillus succinogenes需要添加 CO ₂来生产琥珀酸，这是一种具有多种工业应用的构件。在 24 小时批量实验中测试了两种菌株的不同 CO ₂供应模式，结果与在实验室规模的生物反应器上获得的结果相比较。总体而言，证明微基质是一种有用的按比例缩小工具，适用于在简单的批处理过程中培养代谢不同的菌株，但是，为了充分发挥其潜力，仍需解决一系列问题。