Oridonin is an important diterpenoid, which plays an important role in plant growth and development. PLDα1 and GPA1 are involved in many biotic or abiotic stresses. In this study, using the seedlings of Arabidopsis thaliana L. wild type (WT), PLDα1 defective mutant (pldα1), GPA1 defective mutant (gpa1) and pldα1/gpa1 double mutant as materials, the effect of stomatal apertures responding to Oridonin and the functions of PLDα1 and GPA1 in this response were investigated. The results showed that 60 μmol·L^–1 of Oridonin induced stomatal closure and significantly increased the relative expression levels of GPA1 and PLDα1. Oridonin increased H₂O₂ accumulation in guard cells by inhibiting the antioxidant enzymes. The increase of H₂O₂ caused the expression of OST1, which is a positive regulatory gene for stomatal closure. Both PLDα1 and GPA1 were involved in Oridonin-induced stomatal closure and PLDα1 acted downstream of GPA1. The results suggested that Oridonin caused stomatal closure by affecting GPA1 and promoting PLDα1 to produce PA, and further accumulating H₂O₂ to upregulate gene OST1.

冬凌草素是一种重要的二萜类化合物，在植物生长发育中起重要作用。PLDα1 和 GPA1 参与许多生物或非生物胁迫。本研究以拟南芥野生型（WT）、PLDα1缺陷突变体（pldα1）、GPA1缺陷突变体（gpa1）和pldα1 / gpa1双突变体为材料，研究了气孔孔径对冬凌草素的响应效应和研究了 PLDα1 和 GPA1 在该反应中的功能。结果表明，60 μmol·L ^-1冬凌草素诱导气孔关闭，显着提高GPA1和PLDα1的相对表达水平。. Oridonin 通过抑制抗氧化酶增加了保卫细胞中H ₂ O ₂的积累。H ₂ O ₂的增加引起OST1的表达，OST1是气孔关闭的正调控基因。PLDα1和GPA1都参与了冬凌草素诱导的气孔关闭，PLDα1作用于GPA1的下游。结果表明，冬凌草甲素通过影响GPA1，促进PLDα1产生PA，并进一步积累H ₂ O ₂上调基因OST1而引起气孔关闭。