Abstract Hyaluronic acid (HA) has important applications in fields of health care products, cosmetics and clinical medical. However, the unique physiological properties of HA make cost of its traditional separation and extraction process relatively high. Foam separation technique has simple, gentle and efficient advantages on the separation of substances with surface activity by using bubbles as the separation medium. In this paper, natural surfactant CocamideBetaine (CAPB) was used as a foaming agent to explore the technology of microscale HA in aqueous solution by foam separation. The optimum process conditions were determined based on the recovery rate and enrichment ratio of HA by single factor and orthogonal experiment: at room temperature, pH = 7, separating air velocity (v) = 350 mL/min, HA concentration (CHA) = 50 mg/L, adding liquid volume (V) = 200 mL, collecting time (tcol) = 10 min, CAPB concentration (CCAPB) = 0.035 g/L. Under these conditions, HA enrichment ratio (E) equals 6.821 and HA recovery rate (R) equals 66.425%.

中文翻译：

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泡沫分离技术在水溶液中分离微量HA

摘要 透明质酸（HA）在保健品、化妆品和临床医学领域有着重要的应用。然而，HA独特的生理特性使其传统的分离和提取过程的成本相对较高。泡沫分离技术在以气泡为分离介质分离具有表面活性的物质方面具有简单、温和、高效的优点。本文以天然表面活性剂椰油酰胺甜菜碱（CAPB）为发泡剂，探讨了通过泡沫分离制备水溶液中微量HA的技术。根据HA的回收率和富集率，通过单因素和正交实验确定最佳工艺条件：室温，pH=7，分离风速（v）=350 mL/min，HA浓度（CHA）=50毫克/升，加液量 (V) = 200 mL，收集时间 (tcol) = 10 min，CAPB 浓度 (CCAPB) = 0.035 g/L。在这些条件下，HA 富集率 (E) 等于 6.821，HA 回收率 (R) 等于 66.425%。