The purpose of this study was to investigate cytogenetic variations between the nine Haworthia species necessary for subsequent breeding. Flow cytometric analysis of H. badia ‘Murasaki’, H. splendens, H. truncata ‘Seiko’, H. cooperi var. obtusa, H. cymbiformis, H. mirabilis var. mundula, and H. retusa showed a wide range of DNA content from 10.17 to 12.17 pg/1C, whereas two tetraploids, H. limifolia and H. angustifolia var. baylisii, showed higher DNA content of 16.81 and 12.64 pg/1C, respectively. Seven diploid Haworthia expressed three karyotype formulas, whereas two tetraploid Haworthia each expressed a different karyotype formula. Secondary constriction was observed in H. truncata ‘Seiko’ and H. cooperi var. obtusa. Results of fluorescence in situ hybridization (FISH) showed a major difference between the diploid and tetraploid Haworthia in the distribution of 5S and 18S ribosomal DNA (rDNA) loci. There were eight loci in two out of seven diploids and both tetraploid Haworthia, while H. cymbiformis and H. mirabilis var. mundula each showed seven 18S rDNA loci. H. badia ‘Murasaki’, H. splendens, and H. retusa had four, three, and five 18S rDNA loci, respectively. In addition, a single pair of 5S rDNA loci was observed in all diploid Haworthia except H. cooperi var. obtusa, which had four 5S rDNA loci. Each tetraploid Haworthia had four 5S rDNA loci, with two strong and two weak signals. All 5S and 18S rDNA loci were observed on the long arm, in the subtelomeric or telomeric region, while a significant variation in chromosome length was observed among the Haworthia. This cytological analysis provides the necessary genetic information to elucidate the genetic makeup of newly introduced Haworthia hybrids.

本研究的目的是调查随后育种所需的九种十二卷物种之间的细胞遗传变异。H.badia 'Murasaki'、H. splendens、H. truncata 'Seiko'、H. cooperi var. 的流式细胞术分析。obtusa , H. cymbiformis , H. mirabilis var. mundula和H. retusa的 DNA 含量范围很广，从 10.17 到 12.17 pg/1C，而两个四倍体H. limifolia和H. angustifolia var. 贝利西，分别显示出更高的 DNA 含量，分别为 16.81 和 12.64 pg/1C。七个二倍体十二卷表达了三个核型公式，而两个四倍体十二卷表达了不同的核型公式。在H. truncata 'Seiko' 和H. cooperi var. 中观察到二次收缩。钝器。荧光原位杂交 (FISH) 的结果显示二倍体和四倍体十二卷在 5S 和 18S 核糖体 DNA (rDNA) 基因座的分布方面存在重大差异。七个二倍体中的两个和四倍体十二卷都有八个基因座，而H. cymbiformis和H. mirabilis var.mundula每个显示七个 18S rDNA 基因座。H.badia 'Murasaki'、H. splendens和H. retusa 分别具有四个、三个和五个 18S rDNA 基因座。此外，在除H. cooperi var.之外的所有二倍体十二卷中都观察到了一对 5S rDNA 基因座。obtusa，它有四个 5S rDNA 基因座。每个四倍体十二卷都有四个 5S rDNA 基因座，具有两个强信号和两个弱信号。所有 5S 和 18S rDNA 基因座都在长臂、亚端粒或端粒区域观察到，而在十二卷中观察到染色体长度的显着变化. 这种细胞学分析提供了必要的遗传信息，以阐明新引进的十二卷杂种的基因组成。