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Epigallocatechin gallate and Lactobacillus plantarum culture supernatants exert bactericidal activity and reduce biofilm formation in Clostridium perfringens
Folia Microbiologica ( IF 2.6 ) Pub Date : 2021-06-25 , DOI: 10.1007/s12223-021-00891-z
Alberto Aguayo-Acosta 1 , Eduardo Franco-Frías 1 , Norma Heredia 1 , Jose A Merino-Mascorro 1 , Jorge E Dávila-Aviña 1 , Jorge E Vidal 2 , Santos García 1
Affiliation  

Clostridium perfringens forms biofilms and spores that are a source of food contamination. In this study, the antibacterial activities of Lactobacillus plantarum culture supernatants (LP-S), LP-S fractions, and the plant-derived compound epigallocatechin gallate (EG) were evaluated. Specifically, their effects on the viability and biofilm-forming ability of C. perfringens were assessed. Moreover, the expression of quorum sensing-regulated genes associated with the pathogenesis of this microorganism and that of genes involved in biofilm formation was also investigated. The results showed that both EG and the LP-S exerted bactericidal activity against all C. perfringens strains tested. The minimal bactericidal concentration (MBC) of EG was 75 µg/mL for all strains but ranged from 61 to 121 µg of total protein per mL for LP-S. EG exerted only minor effects on biofilm formation, whereas LP-S, particularly its 10 and 30 K fractions, significantly reduced the biofilm-forming ability of all the strains. The antibiofilm activity of LP-S was lost following preincubation with proteases, suggesting that it was mediated by a proteinaceous molecule. The treatment of C. perfringens with either EG or LP-S did not change the transcript levels of two CpAL (C. perfringens quorum–sensing Agr-like system)-related genes, agrB and agrD, which are known to be involved in the regulation of biofilms, suggesting that LP-S exerted its biofilm inhibitory activity downstream of CpAL signaling. In summary, we demonstrated the bactericidal activity of EG and LP-S against C. perfringens and antibiofilm activity of LP-S at a subinhibitory dose. Our results suggested that these compounds can be further explored for food safety applications to control agents such as C. perfringens.



中文翻译:

表没食子儿茶素没食子酸酯和植物乳杆菌培养上清液在产气荚膜梭菌中发挥杀菌活性并减少生物膜形成

产气荚膜梭菌形成的生物膜和孢子是食物污染的来源。在这项研究中,评估了植物乳杆菌培养上清液 (LP-S)、LP-S 级分和植物来源的化合物表没食子儿茶素没食子酸酯 (EG)的抗菌活性。具体而言,评估了它们对产气荚膜梭菌的生存力和生物膜形成能力的影响。此外,还研究了与该微生物的发病机制相关的群体感应调节基因的表达以及参与生物膜形成的基因的表达。结果表明EG和LP-S对所有产气荚膜梭菌均有杀菌活性菌株测试。对于所有菌株,EG 的最低杀菌浓度 (MBC) 为 75 µg/mL,但对于 LP-S,范围为每毫升 61 至 121 µg 总蛋白。EG 对生物膜形成的影响很小,而 LP-S,尤其是其 10 和 30 K 部分,显着降低了所有菌株的生物膜形成能力。LP-S 的抗生物膜活性在与蛋白酶预孵育后丧失,表明它是由蛋白质分子介导的。用 EG 或 LP-S处理产气荚膜梭菌并没有改变两个 CpAL(产气荚膜梭菌群体感应 Agr 样系统)相关基因agrBagrD的转录水平,已知其参与生物膜的调节,表明 LP-S 在 CpAL 信号传导下游发挥其生物膜抑制活性。总之,我们证明了 EG 和 LP-S 对产气荚膜梭菌的杀菌活性和 LP-S 在亚抑制剂量下的抗生物膜活性。我们的结果表明,可以进一步探索这些化合物在食品安全方面的应用,以控制诸如产气荚膜梭菌之类的药剂。

更新日期:2021-06-25
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