Abstract

Cardiovascular ailments result in a great rate of mortality all over the world. Myocardial infarction is a common presentation of cardiovascular disease. The current work aimed to investigate and compare the cardioprotective potentials of methanolic extracts from the aerial parts from Bauhinia purpurea and Bauhinia madagascariensis in adrenaline-induced cardiotoxicity in rats. The rats were categorized into five groups as follows: control group, adrenaline-treated group, Bauhinia purpurea extract + adrenaline treated group, Bauhinia madagascariensis+ adrenaline treated group, reference drug (captopril) + adrenaline treated group. The extracts as well as the reference drug were orally administered for 21 consecutive days. On day 22, adrenaline was injected as a single dose for 2 consecutive days. The adrenaline injection caused a significant increase (p < 0.05) in serum cardiac markers (ALT, AST, CK-MB, LDH), angiotensin-converting enzyme (ACE) and matrix metalloproteinase (MMP-9), inducible nitric oxide synthase (iNOS) activities, tumor necrosis factor-α (TNF-α) cardiac lipid peroxides (MDA) levels and a significant decline (p < 0.05) in cardiac reduced glutathione (GSH) levels compared to their corresponding controls. The pretreatment extracts significantly ameliorated (p < 0.05) these alterations. Histopathological investigations supported the biochemical data. Bauhinia madagascariensis extract exerted a significant anti-inflammatory activity than that of Bauhinia purpurea. In addition, Bauhinia madagascariensis extract revealed a significant inhibitory activity on ACE compared to that of Bauhinia purpurea, (p < 0.05). These data reveal that both extracts had a strong protective activity against adrenaline-induced cardiotoxicity via improving cardiac function, reducing ECG and histopathological changes that could be mediated in part through its anti-oxidant, anti-inflammatory effects, inhibition of ACE, MMP-9, and iNOS.

摘要

心血管疾病导致全世界的死亡率很高。心肌梗死是心血管疾病的常见表现。目前的工作旨在研究和比较紫荆和马达加斯加洋紫荆地上部分的甲醇提取物对肾上腺素诱导的大鼠心脏毒性的心脏保护作用。将大鼠分为如下五组：对照组、肾上腺素处理组、紫荆提取物+肾上腺素处理组、马达加斯加洋紫荆+肾上腺素治疗组，参考药物（卡托普利）+肾上腺素治疗组。提取物和参考药物连续21天口服给药。在第 22 天，连续 2 天单次注射肾上腺素。肾上腺素注射导致血清心脏标志物（ALT、AST、CK-MB、LDH）、血管紧张素转换酶（ACE）和基质金属蛋白酶（MMP-9）、诱导型一氧化氮合酶（iNOS）显着增加（p < 0.05 ） ) 活性、肿瘤坏死因子-α (TNF-α) 心脏脂质过氧化物 (MDA) 水平和心脏还原型谷胱甘肽 (GSH) 水平显着下降 ( p < 0.05 ) 与相应的对照相比。预处理提取物显着改善 ( p < 0.05) 这些改动。组织病理学研究支持生化数据。马达加斯加洋紫荆提取物比紫荆花具有显着的抗炎活性。此外，与紫荆花相比，马达加斯加洋紫荆提取物对 ACE 具有显着的抑制活性（p < 0.05）。这些数据表明，两种提取物通过改善心脏功能、减少心电图和组织病理学变化对肾上腺素诱导的心脏毒性具有很强的保护活性，这可能部分通过其抗氧化、抗炎作用、抑制 ACE、MMP-9 来介导, 和 iNOS。