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Multichannel near-infrared spectroscopy brain imaging system for small animals in mobile conditions
Neurophotonics ( IF 5.3 ) Pub Date : 2021-06-01 , DOI: 10.1117/1.nph.8.2.025013
Seung-Ho Paik 1, 2 , Seung Hyun Lee 1 , Ju-Hee Kim 3 , Shin-Young Kang 3 , Zephaniah Phillips V 3 , Youngwoon Choi 3 , Beop-Min Kim 3, 4
Affiliation  

Significance: We propose a customized animal-specific head cap and an near-infrared spectroscopy (NIRS) system to obtain NIRS signals in mobile small animals. NIRS studies in mobile small animals provide a feasible solution for comprehensive brain function studies. Aim: We aim to develop and validate a multichannel NIRS system capable of performing functional brain imaging along with a closed-box stimulation kit for small animals in mobile conditions. Approach: The customized NIRS system uses light-weight long optical fibers, along with a customized light-weight head cap to securely attach the optical fibers to the mouse. A customized stimulation box was designed to perform various stimuli in a controlled environment. The system performance was tested in a visual stimulation task on eight anesthetized mice and eight freely moving mice. Results: Following the visual stimulation task, we observed a significant stimulation-related oxyhemoglobin (HbO) increase in the visual cortex of freely moving mice during the task. In contrast, HbO concentration did not change significantly in the visual cortex of anesthetized mice. Conclusions: We demonstrate the feasibility of a wearable, multichannel NIRS system for small animals in a less confined experimental design.

中文翻译:

移动条件下小动物多通道近红外光谱脑成像系统

意义:我们提出了一种定制的动物专用头帽和近红外光谱(NIRS)系统来获取移动小动物的近红外光谱信号。移动小动物的 NIRS 研究为全面的脑功能研究提供了可行的解决方案。目标:我们的目标是开发和验证多通道 NIRS 系统,该系统能够在移动条件下为小动物执行功能性脑成像以及封闭盒刺激套件。方法:定制的 NIRS 系统使用轻型长光纤,以及定制的轻型头帽,将光纤牢固地连接到鼠标。定制的刺激盒旨在在受控环境中执行各种刺激。系统性能在 8 只麻醉小鼠和 8 只自由移动的小鼠的视觉刺激任务中进行了测试。结果:在视觉刺激任务之后,我们观察到在任务期间自由移动的小鼠的视觉皮层中与刺激相关的氧合血红蛋白 (HbO) 显着增加。相比之下,麻醉小鼠的视觉皮层中的 HbO 浓度没有显着变化。结论:我们证明了适用于小动物的可穿戴多通道 NIRS 系统在限制较少的实验设计中的可行性。
更新日期:2021-06-25
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