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Biosynthesis of polyhydroxyalkanoate from food waste oil by Pseudomonas alcaligenes with simultaneous energy recovery from fermentation wastewater
Waste Management ( IF 8.1 ) Pub Date : 2021-06-25 , DOI: 10.1016/j.wasman.2021.06.008
Lanjia Pan 1 , Jie Li 2 , Ruming Wang 3 , Yu Wang 3 , Qinghuai Lin 4 , Chunxing Li 5 , Yin Wang 6
Affiliation  

Bioconversion of food waste oil (FWO) into biodegradable plastic is a promising method for converting waste into high-value products. In this study, a strain (Pseudomonas sp. H3) was isolated for polyhydroxyalkanoate (PHA) synthesis from FWO. After 72 h of cultivation with 20 g/L of FWO, the high cell dry weight (CDW) of 3.6 g/L, PHA yield of 2.4 g/L, and PHA content of 65 wt% were obtained under the optimal temperature (25 °C) and inoculum amount (6% (v/v)). Fed-batch fermentation was conducted in a 5 L bioreactor with a maximum CDW of 16 g/L, PHA content of 54 wt%, and PHA productivity of 0.23 g/(L·h) after 36 h. The PHA had a molecular weight of 54 782 Da and a low polydispersity index of 1.41 with glass transition, melting, and degradation temperatures of −20 °C, 34 °C, and 210 °C, respectively. To further utilize the wastewater after PHA production, anaerobic digestion was employed for CH4 production, and the CH4 yield was 284 mL/g volatile solids. Microbial community analysis showed that the abundance of acetate-oxidizing bacteria and Methanobacterium significantly increased during anaerobic digestion. This study describes a new strain for the economical synthesis of biodegradable plastics and presents a novel framework for fully utilizing FWO with the production of PHA and CH4.



中文翻译:

产碱假单胞菌从食品废油中生物合成聚羟基链烷酸酯并同时从发酵废水中回收能量

将食物废油 (FWO) 生物转化为可生物降解塑料是将废物转化为高价值产品的一种很有前景的方法。在本研究中,一株(假单胞菌sp. H3) 被分离用于从 FWO 合成聚羟基链烷酸酯 (PHA)。20 g/L FWO培养72 h后,在最适温度(25 °C) 和接种量 (6% (v/v))。补料分批发酵在 5 L 生物反应器中进行,最大 CDW 为 16 g/L,PHA 含量为 54 wt%,36 h 后 PHA 生产率为 0.23 g/(L·h)。PHA 的分子量为 54 782 Da,低多分散指数为 1.41,玻璃化转变温度、熔融温度和降解温度分别为 -20 °C、34 °C 和 210 °C。为了进一步利用 PHA 生产后的废水,CH 4生产采用厌氧消化,CH 4产量为284mL/g挥发性固体。微生物群落分析表明,厌氧消化过程中乙酸氧化细菌甲烷杆菌的丰度显着增加。本研究描述了一种用于经济合成可生物降解塑料的新菌株,并提出了一种新框架,可充分利用 FWO 生产 PHA 和 CH 4

更新日期:2021-06-25
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