The usage of natural sources like lichen extracts and their metabolites as anti-inflammatory agents is well known for ages. Based on the data of the folklore and documented books, researchers tested lichens and their products for anti-inflammatory properties and they identified many therapeutic agents used to diagnosis acute and chronic inflammation with lesser side effects. In this study, we aimed to examine the acetone extract of Cladonia corniculata(AE)and its metabolites for in vitro anti-inflammatory and anti-gout effects. Through chemical investigation, we have successfully isolated and identified three known dibenzofurans, namely alectosarmentin 1, porphyrilic acid 2, and strepsilin 3 from the AE. All isolated dibenzofurans1-3 showed prominent inhibition of cyclooxygenase enzymes, whereas compounds 1 and 2 exhibited noticeable inhibition of 5-lipoxygenase enzyme. Only compound 2 showed significant inhibition of xanthine oxidase enzyme with an IC₅₀ value of 80.17±0.66 µg/mL, while standard drug allopurinol with 9.10±0.64 µg/mL. The results indicate that C. corniculatacan be a favourable natural source for the treatment of inflammation and gout and these actions are linked to the natural active dibenzofurans1-3.

中文翻译：

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来自 Cladonia corniculata Ahti 和 Kashiw 的二苯并呋喃抑制炎症和痛风中涉及的关键酶：一种体外方法

地衣提取物及其代谢物等天然来源作为抗炎剂的用途早已广为人知。根据民间传说和文献记载的数据，研究人员测试了地衣及其产品的抗炎特性，他们确定了许多用于诊断急性和慢性炎症且副作用较小的治疗剂。在本研究中，我们的目的是检查小花枝条(AE)的丙酮提取物及其代谢物的体外抗炎和抗痛风作用。通过化学研究，我们成功分离并鉴定了三种已知的二苯并呋喃，即alectosarmentin 1、卟啉酸2和strepsilin 3来自AE。所有分离的二苯并呋喃1-3均显示出对环氧合酶的显着抑制，而化合物1和2对 5-脂加氧酶显示出明显的抑制。只有化合物2显示出对黄嘌呤氧化酶的显着抑制，IC ₅₀值为 80.17±0.66 µg/mL，而标准药物别嘌呤醇为 9.10±0.64 µg/mL。结果表明，C.corniculata可以成为治疗炎症和痛风的有利天然来源，这些作用与天然活性二苯并呋喃有关1-3。