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Comparative liquid chromatography/tandem mass spectrometry lipidomics analysis of macaque heart tissue flash-frozen or embedded in optimal cutting temperature polymer (OCT): Practical considerations
Rapid Communications in Mass Spectrometry ( IF 2 ) Pub Date : 2021-06-25 , DOI: 10.1002/rcm.9155
Ashish Vaswani 1 , Armando Alcazar Magana 2 , Eric Zimmermann 3 , Wohaib Hasan 4 , Jaishankar Raman 5 , Claudia S Maier 1, 2
Affiliation  

Biobanks of patient tissues have emerged as essential resources in biomedical research. Optimal cutting temperature compound (OCT) blends have shown to provide stability to the embedded tissue and are compatible with spectroscopic methods, such as infrared (IR) and Raman spectroscopy. Data derived from omics-methods are only useful if tissue damage caused by storage in OCT blends is minimal and well understood. In this context, we investigated the suitability of OCT storage for heart tissue destined for liquid chromatography/tandem mass spectrometry (LC/MS/MS) lipidomic studies.

中文翻译:

猕猴心脏组织快速冷冻或嵌入最佳切割温度聚合物 (OCT) 的比较液相色谱/串联质谱脂质组学分析:实际考虑

患者组织的生物库已成为生物医学研究的重要资源。最佳切割温度化合物 (OCT) 混合物已显示为嵌入组织提供稳定性,并与光谱方法兼容,例如红外 (IR) 和拉曼光谱。来自组学方法的数据只有在 OCT 混合物中存储造成的组织损伤最小且易于理解时才有用。在此背景下,我们研究了用于液相色谱/串联质谱 (LC/MS/MS) 脂质组学研究的心脏组织的 OCT 存储的适用性。
更新日期:2021-07-16
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