Optical coherence tomography (OCT) is an imaging modality that provides real-time visualization of tissue microstructure. The goals of this study were to assess OCT image tissue depths (TD) with image processing, and other objective characteristics of tissue types at surgical margins in canine soft tissue sarcoma (STS). In this study, a single observer reviewed 248 images of four tissue types (sarcoma, skeletal muscle, adipose and fascia) collected from 24 dogs with STS. The observer evaluated for tissue characteristics and measured TDs utilizing ImageJ software. Images were evaluated in normal, Threshold and Binary conditions. Measurements were repeated 1 week later to evaluate for intra-observer variability. In all three image processing conditions, the order of TD from greatest to least light penetration was adipose, skeletal muscle, fascia and sarcoma tissue. Neovascularization was observed in 53.2% sarcoma, and lines of fascia surrounding muscle bundles was present in 93.5% of skeletal muscle images. These observed differences between tissue types in OCT images can be utilized to improve observer evaluation, aid in development of algorithms, and improve the accuracy of surgical margin assessment.

中文翻译：

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探索光学相干断层扫描成像深度以区分手术边缘的组织

光学相干断层扫描 (OCT) 是一种成像模式，可提供组织微观结构的实时可视化。本研究的目的是通过图像处理评估 OCT 图像组织深度 (TD)，以及犬软组织肉瘤 (STS) 手术边缘组织类型的其他客观特征。在这项研究中，一名观察者审查了从 24 只患有 STS 的狗收集的四种组织类型（肉瘤、骨骼肌、脂肪和筋膜）的 248 张图像。观察者使用 ImageJ 软件评估组织特征并测量 TD。在正常、阈值和二进制条件下评估图像。1 周后重复测量以评估观察者内的变异性。在所有三种图像处理条件下，光穿透率从最大到最小的 TD 顺序是脂肪、骨骼肌、筋膜和肉瘤组织。在 53.2% 的肉瘤中观察到新血管形成，并且在 93.5% 的骨骼肌图像中存在围绕肌束的筋膜线。这些在 OCT 图像中观察到的组织类型之间的差异可用于改进观察者评估、帮助开发算法并提高手术切缘评估的准确性。