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A new antidiabetic foot bacteria formula from marine chitosan nanosilver-metal complex
Environmental Science and Pollution Research ( IF 5.8 ) Pub Date : 2021-06-24 , DOI: 10.1007/s11356-021-14958-4
Hasnaa E-B Ghonam 1 , Morsy A Abu Yousef 2 , Yousry M Gohar 3 , Rafa Almeer 4 , Khouloud M Barakat 1
Affiliation  

In more than two-thirds of the diabetic foot ulcer (DFUs) cases, lower limb amputation of foot ulceration is caused by the infection. The role of transition a metal complex as a therapeutic compound is becoming increasingly important. In vitro, four groups of antibiotics and one sulfa drug were tested against diabetic foot resistant bacteria. Using three concentrations of two different prepared metal complexes: copper (Cu) and silver (Ag) — isoniazid (Iso) and nicotinamide (Nicot) were tested against diabetic foot isolates. Results revealed that β-lactam drugs (cephradine and piperacillin) showed the minimum averages of MIC 265 μg/ml against Gram-positive and Gram-negative isolates. Silver isoniazid (Iso-Ag-1) metal complex was selected depending on the maximum averages of MIC against both types of clinical isolates. The combination between β-lactams and Iso-Ag-1 showed maximum FICI averages of 0.24 for Gram-positive and 0.28 for Gram-negative. In addition, a combination between Iso-Ag-1 with squilla chitosan nanoparticles (CSSq-nAg) showed averages of synergistic index by 0.23 against Staphylococcus aureus and 0.13, 0.30, and 0.27 against E. coli, K. pneumoniae, and Ps. aeruginosa, respectively. Final formula of Iso-Ag-1+CSSq-nAg + β-lactams (cephradine and piperacillin) showed a synergistic effect at FICI = 0.044 and 0.047, against G+ve and –ve, respectively. These two combinations showed a slight toxicity against the water flea Daphnia magna by 3.49 and 3.6 ppm, respectively. Results suggest the use of Iso-Ag-1-CSSq-nAg as enhancing agent in combination with β-lactams as a blind therapy in pharmaceutical preparations.



中文翻译:

一种来自海洋壳聚糖纳米银-金属复合物的抗糖尿病足部细菌新配方

在超过三分之二的糖尿病足溃疡 (DFU) 病例中,足部溃疡的下肢截肢是由感染引起的。过渡金属络合物作为治疗化合物的作用变得越来越重要。在体外,四组抗生素和一种磺胺药物针对糖尿病足耐药菌进行了测试。使用三种浓度的两种不同制备的金属络合物:铜 (Cu) 和银 (Ag) — 异烟肼 (Iso) 和烟酰胺 (Nicot) 对糖尿病足分离株进行了测试。结果显示,β-内酰胺类药物(头孢拉定和哌拉西林)对革兰氏阳性和革兰氏阴性分离株的最低平均 MIC 值为 265 μg/ml。银异烟肼 (Iso-Ag-1) 金属络合物的选择取决于对两种临床分离株的最大 MIC 平均值。β-内酰胺和 Iso-Ag-1 的组合显示出革兰氏阳性的最大 FICI 平均值为 0.24,革兰氏阴性的最大 FICI 平均值为 0.28。此外,Iso-Ag-1 与乌贼壳聚糖纳米粒子 (CSSq-nAg) 的组合显示协同指数的平均值为 0.23金黄色葡萄球菌和 0.13、0.30 和 0.27 对大肠杆菌肺炎克雷伯菌Ps。铜绿假单胞菌,分别。Iso-Ag-1+CSSq-nAg + β-内酰胺类(头孢拉定和哌拉西林)的最终配方在 FICI = 0.044 和 0.047 时分别显示出对 G+ve 和 -ve 的协同作用。这两种组合对水蚤Daphnia magna的毒性分别为 3.49 ppm 和 3.6 ppm。结果表明使用 Iso-Ag-1-CSSq-nAg 作为增强剂与 β-内酰胺类药物组合作为药物制剂中的盲法治疗。

更新日期:2021-06-24
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