A 60-day feeding trial was conducted to investigate the effect of enzymatic hydrolysis chicken by-product (EHC) supplementation in high plant protein diets on growth performance, digestive capacity, antioxidant capacity and non-specific immunity of juvenile turbot (Scophthalmus maximus L.). The diet with 620 g/kg fish meal (FM) was used as the positive control group (FM group), while the diet with 372 g/kg FM and 256 g/kg degossypolled cottonseed protein were used as the negative control group (0 g/kg EHC group). Based on the negative control diet, grade levels of EHC (10, 20, 30 and 40 g/kg) were added, respectively. Results showed that fish fed with a high plant protein diet showed significantly lower growth performance, digestive capacity, antioxidant capacity and non-specific immunity than those of the positive control. The supplementation of 20 g/kg EHC significantly increased growth performance of fish as compared to the negative control. Furthermore, the digestive capacity of turbot fed the diet with 20 g/kg EHC was significantly improved by increasing activities of trypsin, pepsin and lipase and the expression of peptide transporter 1 (pept1). The antioxidant capacity and non-specific immunity of fish fed the diet with 20, 30 and 40 g/kg EHC were significantly improved by increasing activities of superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant capacity (T-AOC), lysozyme (LZM) and acid phosphatase (ACP) in serum, decreasing the expression of interleukin-1β (il-1β) and tumour necrosis factor-α (tnf-α) and increasing the expression of transforming growth factor-β (tgf-β). In conclusion, the adverse effect of high plant protein diet on growth performance of turbot could be improved with the supplementation of 20 g/kg EHC, which could be due to the positive effect of EHC on digestive capacity, antioxidant capacity and non-specific immunity of juvenile turbot.

中文翻译：

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酶解鸡副产物在高植物蛋白日粮中对大菱鲆幼鱼生长性能、消化能力、抗氧化能力和非特异性免疫的影响

60天喂养试验以调查酶促水解鸡的副产物的效果对生长性能（EHC）补充在高植物蛋白质饮食，消化能力，抗氧化能力和大菱鲆幼鱼的非特异性免疫（鲆L.）。以620 g/kg鱼粉(FM)日粮为阳性对照组(FM组)，以372 g/kg FM和256 g/kg去棉籽蛋白日粮为阴性对照组(0 g/kg EHC 组）。基于阴性对照饮食，分别添加 EHC 等级水平（10、20、30 和 40 g/kg）。结果表明，与阳性对照相比，饲喂高植物蛋白饲料的鱼的生长性能、消化能力、抗氧化能力和非特异性免疫显着降低。与阴性对照相比，添加 20 g/kg EHC 显着提高了鱼的生长性能。此外，通过增加胰蛋白酶的活性，大菱鲆的消化能力显着提高，其中含有 20 g/kg EHC 的饮食，pept1 )。通过增加超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GPX)、总抗氧化能力 (T-AOC) 的活性，鱼的抗氧化能力和非特异性免疫能力显着提高，饲喂 20、30 和 40 g/kg EHC 、血清中溶菌酶（LZM）和酸性磷酸酶（ACP），降低白细胞介素-1β（il-1β）和肿瘤坏死因子-α（tnf-α）的表达，增加转化生长因子-β（tgf- β）。综上所述，高植物蛋白日粮对大菱鲆生长性能的不利影响可以通过补充20 g/kg EHC得到改善，这可能是由于EHC对消化能力、抗氧化能力和非特异性免疫的积极作用的少年大菱鲆。