当前位置: X-MOL 学术Rice Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A New Approach to Select Doubled Haploid Rice Lines under Salinity Stress Using Indirect Selection Index
Rice Science ( IF 4.8 ) Pub Date : 2021-06-23 , DOI: 10.1016/j.rsci.2021.05.007
Muhammad Fuad Anshori , Bambang Sapta Purwoko , Iswari Saraswati Dewi , Sintho Wahyuning Ardie , Willy Bayuardi Suwarno

This study determined the indirect selection index of doubled haploid (DH) rice using a multivariate analysis approach to select lines adaptive to salinity stress, comprising three experiments. The first experiment involved the selection of good agronomic characters in a field experiment conducted at an experimental station in Bogor, Indonesia. The second experiment involved salinity tolerance screening through hydroponic cultures using 0 and 120 mmol/L NaCl, conducted at a greenhouse in Bogor. The third experiment involved the validation of the indirect adaptability selection index (IASI) through a field experiment in Sukra (saline area). Field experiments followed a randomized complete block design (RCBD), whereas an RCBD nested factorial design was used for the greenhouse experiment. The first and second experiments used 56 DH lines and four check varieties with three replications. In the second experiment, Pokkali and IR29 varieties were also added as tolerant and sensitive checks of salinity, respectively. The third experiment used 28 selected DH lines, Inpari 29 and one sensitive DH line. The good agronomic index (GAI) was 0.465 yield + 0.433 number of productive tillers + 0.31 number of filled grains. This generated 24 DH rice lines with good agronomic traits. The salinity tolerance index (SaTI) was developed through the average of standardized salinity tolerance score and salinity selection index based on discriminant analysis. This generated 34 DH rice lines with good salinity stress tolerance. The IASI (IASI = GAI – SaTI) selected 28 DH rice lines adaptive to salinity stress and it was considered effective by Sukra validation.



中文翻译:

利用间接选择指数筛选盐胁迫下双单倍体水稻品系的新方法

本研究使用多变量分析方法选择适应盐度胁迫的品系,确定了双单倍体 (DH) 水稻的间接选择指数,包括三个实验。第一个试验涉及在印度尼西亚茂物的一个试验站进行的田间试验中选择良好的农艺性状。第二个实验涉及通过使用 0 和 120 mmol/L NaCl 的水培培养物进行耐盐性筛选,在茂物的温室中进行。第三个实验涉及通过在 Sukra(盐碱区)的田间实验验证间接适应性选择指数 (IASI)。田间试验采用随机完全区组设计 (RCBD),而温室试验采用 RCBD 嵌套因子设计。第一个和第二个实验使用 56 个 DH 品系和四个检查品种,三个重复。在第二个实验中,还分别添加了 Pokkali 和 IR29 品种作为盐度的耐受性和敏感性检查。第三个实验使用了 28 个选定的 DH 系,Inpari 29 和一个敏感的 DH 系。优良农艺指数 (GAI) 为 0.465 +  0.433 分蘖数量 +  0.31 饱满谷物数量。这产生了 24 个具有良好农艺性状的 DH 水稻品系。盐度耐受指数(SaTI)是通过基于判别分析的标准化耐盐度得分和盐度选择指数的平均值制定的。这产生了具有良好盐胁迫耐受性的 34 个 DH 水稻品系。IASI ( IASI  = GAI – Sa TI ) 选择了 28 个适应盐度胁迫的 DH 水稻品系,Sukra 验证认为它是有效的。 

更新日期:2021-06-24
down
wechat
bug