Methicillin resistant Staphylococcus aureus (MRSA) is a notorious pathogen which causes severe food borne infections. The formation of biofilms and virulence factors by MRSA mediates the risk of infections. Bacteria living in biofilm communities are highly resistant to current day available drugs and preservatives because the extracellular matrix (ECM) present in the biofilms sequesters the antimicrobial agents. The hesitance of consumers to procure antibiotic treated food products and its associated antimicrobial resistance have pushed the food industry to look for antibiotic free food preservatives. The present study investigated the antibiofilm efficacy of jacalin copper sulfide nanoparticles (JCuS NPs) against MRSA biofilms. The outcome of the study shows that there was significant inhibition in young biofilms and disruption of mature biofilms which is evident by light microscopy and SEM micrographs. Slime synthesis, which is one of the major virulence factors of MRSA, was inhibited upon treatment with JCuS NPs. In addition to antibiofilm efficacy, the food preservative effect of JCuS NPs was evaluated in meat samples (chicken) by thiobarbituric acid reactive substances (TBARS) assay which showed that there was a significant inhibition in lipid peroxidation. Real time PCR analysis of biofilm and virulence associated genes were analyzed for their expression upon treatment. The results conclude that JCuS NPs exhibited enhanced biofilm inhibition potential when compared to CuS NPs alone. Furthermore, JCuS NPs disrupted the biofilms formed on the poultry meat sample with good lipid peroxidation inhibition efficacy. The overall finding reveals that JCuS NPs can be used as a food preservative to extend the shelf life of meat products.

中文翻译：

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Jacalin 水胶体纳米缀合物可减轻肉制品上的耐甲氧西林金黄色葡萄球菌(MRSA) 生物膜

耐甲氧西林金黄色葡萄球菌(MRSA) 是一种臭名昭著的病原体，可导致严重的食源性感染。MRSA 形成的生物膜和毒力因子介导了感染的风险。生活在生物膜群落中的细菌对当今可用的药物和防腐剂具有高度抗性，因为生物膜中存在的细胞外基质 (ECM) 隔离了抗菌剂。消费者对购买经过抗生素处理的食品的犹豫及其相关的抗菌素耐药性促使食品行业寻找不含抗生素的食品防腐剂。本研究调查了 jacalin 硫化铜纳米粒子 (JCuS NPs) 对 MRSA 生物膜的抗生物膜功效。研究结果表明，光学显微镜和 SEM 显微照片表明，对年轻生物膜和成熟生物膜的破坏有显着的抑制作用。粘液合成是 MRSA 的主要毒力因子之一，在用 JCuS NPs 处理后受到抑制。除了抗生物膜功效外，还通过硫代巴比妥酸反应性物质 (TBARS) 测定法在肉类样品（鸡肉）中评估了 JCuS NPs 的食品防腐效果，结果表明对脂质过氧化有显着抑制作用。对生物膜和毒力相关基因的实时 PCR 分析分析了它们在治疗时的表达。结果得出结论，与单独的 CuS NPs 相比，JCuS NPs 表现出增强的生物膜抑制潜力。此外，JCuS NPs 破坏了禽肉样品上形成的生物膜，具有良好的脂质过氧化抑制功效。总体发现表明，JCuS NPs 可用作食品防腐剂，以延长肉制品的保质期。