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Vacuolar transporter Mnr2 safeguards organellar integrity in aged cells
Molecular Microbiology ( IF 3.6 ) Pub Date : 2021-06-24 , DOI: 10.1111/mmi.14776
Md Hashim Reza 1, 2 , Rajesh Patkar 2, 3 , Kaustuv Sanyal 1
Affiliation  

Aging is associated with altered mitochondrial function, which is dependent on the magnesium (Mg+2) ion flux. The molecular mechanism underlying Mg+2 homeostasis, especially during aging has not been well understood. We previously demonstrated that the absence of a vacuolar ion transporter Mnr2 accelerates cell death in the older part of the colony in Magnaporthe oryzae presumably due to an altered Mg+2 homeostasis. Here, we show the localization of Mnr2 as dynamic puncta at the vacuolar membrane, especially in the older Magnaporthe cells. Such vacuolar Mnr2 puncta are often localized in close proximity with the filamentous mitochondria in the older cells. Further, we show loss of integrity of mitochondria and vacuoles in older mnr2∆ null cells. Remarkably, exogenously added Mg+2 restores the mitochondrial structure as well as improves the lifespan of mnr2∆ null cells. Taken together, we propose an ion transporter Mnr2-based Mg+2 homeostasis as a means in preserving mitochondrial and vacuolar integrity and function in older M. oryzae cells.

中文翻译:

液泡转运蛋白 Mnr2 保护衰老细胞的细胞器完整性

衰老与线粒体功能改变有关,这取决于镁 (Mg +2 ) 离子通量。Mg +2稳态的分子机制,特别是在老化过程中,尚未得到很好的理解。我们之前已经证明,空泡离子转运蛋白 Mnr2 的缺失会加速稻瘟病菌中较老部分的细胞死亡,这可能是由于 Mg +2稳态的改变。在这里,我们将 Mnr2 定位为液泡膜上的动态斑点,尤其是在较老的Magnaporthe细胞。这种液泡状的 Mnr2 斑点通常位于较老细胞中的丝状线粒体附近。此外,我们发现旧的mnr2 Δ空细胞中线粒体和液泡的完整性丧失。值得注意的是,外源添加的 Mg +2恢复了线粒体结构并提高了mnr2 Δ null 细胞的寿命。总之,我们提出了一种基于离子转运蛋白 Mnr2 的 Mg +2稳态,作为在较老的分枝杆菌细胞中保持线粒体和液泡完整性和功能的一种手段。
更新日期:2021-06-24
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