当前位置: X-MOL 学术Limnol. Oceanogr. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Bacterioplankton respond with similar transcriptional activity to allochthonous dissolved organic matter in coastal and offshore Lake Michigan
Limnology and Oceanography ( IF 4.5 ) Pub Date : 2021-06-24 , DOI: 10.1002/lno.11869
Adit Chaudhary 1 , Sarah Turner 1 , Rachel Poretsky 1
Affiliation  

Lake Michigan is one of the largest lakes in the world and the second largest of the North American Great Lakes by volume. Over the last two decades, the lake has witnessed significant ecological changes due to proliferation of invasive quagga mussels into its deeper offshore regions. The impact of these changes on the labile dissolved organic matter pool available for bacterial consumption and the relative importance of terrestrially derived DOM (t-DOM) for bacterial metabolism across Lake Michigan in the post-mussel period is poorly understood. Here, we investigated Lake Michigan bacterial community structure and activity in offshore and coastal regions near the mouth of Kalamazoo River, one of the largest tributaries to southern Lake Michigan. In addition, we evaluated short-term bacterioplankton response to a pulse of t-DOM (leaf litter leachate) in shipboard mesocosms set up using nearshore and offshore lake-water. The bacterial community composition and activity for the natural and t-DOM enriched samples were characterized using combined metagenomics and metatranscriptomics. We observed four-fold more transcripts for Cyanobacteria in the offshore bacterial community compared to nearshore, highlighting the importance of Cyanobacterial primary production in supporting microbial food web of the oligotrophic offshore. However, despite this and certain differences in DOM related transporter gene transcripts between the two regions, the nearshore and offshore bacterial communities showed a similar response to t-DOM, primarily in the form of increased transcriptional activity for aromatic compound metabolism. The use of metagenome assembled genomes identified populations within the Bacteroidetes phylum that play an important role in t-DOM response.

中文翻译:

浮游细菌对密歇根湖沿岸和近海的外来溶解有机物具有相似的转录活性

密歇根湖是世界上最大的湖泊之一,也是北美五大湖中体积第二大的湖泊。在过去的二十年里,由于入侵斑驴向其更深的近海区域扩散,该湖见证了重大的生态变化。这些变化对可用于细菌消耗的不稳定溶解有机物池的影响以及陆源 DOM (t-DOM) 对后贻贝时期密歇根湖细菌代谢的相对重要性知之甚少。在这里,我们调查了密歇根湖南部最大支流之一卡拉马祖河河口附近的近海和沿海地区的密歇根湖细菌群落结构和活动。此外,我们评估了浮游细菌对使用近岸和近海湖水设置的船上中观环境中 t-DOM(落叶渗滤液)脉冲的短期反应。使用组合宏基因组学和宏转录组学表征天然和富含 t-DOM 的样品的细菌群落组成和活性。我们观察到离岸细菌群落中蓝藻的转录本比近岸多四倍,突出了蓝藻初级生产在支持寡营养近海微生物食物网方面的重要性。然而,尽管这两个区域之间 DOM 相关转运蛋白基因转录本存在某些差异,但近岸和近海细菌群落对 t-DOM 表现出相似的反应,主要以增加芳香族化合物代谢的转录活性的形式。使用宏基因组组装的基因组确定了拟杆菌门内的种群,这些种群在 t-DOM 响应中起重要作用。
更新日期:2021-08-07
down
wechat
bug