One of the major issues in the control of type 2 diabetes is to reduce hyperglycemia after sugar intake, which is possible through inhibition of enzymes such as α-amylase. In this study, we decided to investigate the inhibitory effect of plant extracts of Salvia leriifolia (Norouzak) on bacterial α-amylase and pancreatic enzymes. The results indicated that hexan and methanol extracts of S. leriifolia had the strongest α-amylase inhibitory effect with IC₅₀ values of 25.6 and 30.5 mg/ml, respectively. The results exhibited a competitive inhibition kinetics towards starch as a substrate for both α-amylases. The fluorescence spectrum of the enzyme revealed that the enzyme structure was opened in the presence of herb extracts. Using the DPPH assay, antioxidant activity was also measured. Antibacterial effect of plant extract was tested against three bacteria, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli; furthermore, MIC and MBC values were determined for each bacteria by the microdilution broth method. The maximum inhibitory effect was observed in extracts of the bacterium P. aeruginosa with MIC = 80 mg/ml. Folin–Siocalcho reagent was used to measure the phenol of extract, and the amount of flavonoids was determined based on colorimetric aluminum chloride method. Statistical analysis using SPSS depicted that the phenol and flavonoid contents were significantly correlated with the antioxidant activity of the plant. According to the amount of IC₅₀ compared with synthetic antioxidants, Norouzak is suitable for using as treatment or a natural preservative to replace the synthetic variety.

控制 2 型糖尿病的主要问题之一是减少摄入糖分后的高血糖，这可以通过抑制 α-淀粉酶等酶来实现。在本研究中，我们决定研究丹参(Norouzak)植物提取物对细菌 α-淀粉酶和胰酶的抑制作用。结果表明，莲子的己正和甲醇提取物对α-淀粉酶的抑制作用最强，IC ₅₀值分别为 25.6 和 30.5 毫克/毫升。结果显示出对淀粉作为两种 α-淀粉酶的底物的竞争性抑制动力学。酶的荧光光谱显示酶结构在草本提取物存在下打开。使用 DPPH 测定，还测量了抗氧化活性。测试植物提取物对金黄色葡萄球菌、铜绿假单胞菌和大肠杆菌三种细菌的抗菌作用；此外，通过微量稀释肉汤法测定每种细菌的 MIC 和 MBC 值。在铜绿假单胞菌的提取物中观察到最大的抑制作用MIC = 80 毫克/毫升。Folin-Siocalcho试剂用于测定提取物中的酚，黄酮含量采用氯化铝比色法测定。使用 SPSS 进行统计分析表明，苯酚和类黄酮含量与植物的抗氧化活性显着相关。根据IC ₅₀与合成抗氧化剂的含量相比，Norouzak 适合用作处理或天然防腐剂以替代合成品种。