Background

Inonotus obliquus, a famous dark brown white-rot fungus on birch trees, has remarkable anti-cancer activity due to its special triterpenpoid components. The aims of this study were to develop an efficient and integrated strategy for preparative separation of triterpenoids from Inonotus obliquus and to provide ideas for the preparation of bioactive ingredients in other complex materials.

Methods

In the present study, an integrated technology of negative pressure cavitation extraction (NPCE) and high-speed counter-current chromatography (HSCCC) separation of four main triterpenoids in Inonotus obliquus was developed for the first time. Response surface methodology was used to optimize the negative pressure cavitation extraction parameters of triterpenoids from Inonotus obliquus. For the rapid and effective preparative separation of triterpenoids, the HSCCC method with Hex-ACN (1:1; v/v) system was established.

Significant Findings

The extraction yield of total triterpenoids reached 17.41 mg/g under the optimal NPCE extraction conditions. The specific four triterpenoids including 3β-hydroxy-lanosta-8, 24-diene-21-al (0.84 mg/g), Inoterpene F (1.09 mg/g), Inotodiol (2.93 mg/g), Lanosterol (1.42 mg/g) were obtained by one step separation with the purities of more than 95%. This is the first report on the preparative separation of triterpenoids from Inonotus obliquus by NPCE-HSCCC.

中文翻译：

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基于负压空化萃取和高速逆流色谱法制备分离木犀草特定三萜类化合物

背景

桦褐孔菌，著名的黑褐色白腐菌的桦树，具有显着的抗肿瘤活性，由于其特殊的triterpenpoid组件。本研究的目的是开发一种有效的综合策略，用于从Innonotus obliquus 中制备三萜类化合物，并为其他复杂材料中生物活性成分的制备提供思路。

方法

在本研究中，首次开发了负压空化萃取 (NPCE) 和高速逆流色谱 (HSCCC) 分离桦褐孔菌中四种主要三萜类化合物的集成技术。采用响应面法优化了木犀草中三萜类化合物的负压空化提取参数。为了快速有效地分离三萜类化合物，建立了 HSCCC 方法和 Hex-ACN (1:1; v/v) 系统。

重要发现

在最佳NPCE提取条件下，总三萜提取率达到17.41 mg/g。具体的四种三萜包括 3β-羟基-lanosta-8、24-diene-21-al (0.84 mg/g)、Inotoperne F (1.09 mg/g)、Inotodiol (2.93 mg/g)、羊毛甾醇 (1.42 mg/g) )通过一步分离获得，纯度超过95%。这是NPCE-HSCCC从Innonotus obliquus 中分离出三萜类化合物的第一份报告。