Biochemicals production from the energy crop, sweet sorghum [Sorghum bicolor (L.) Moench], has attracted wide attention. However, utilization of sweet sorghum for phenolic compounds production has not yet been reported. To improve comprehensive utilization of sweet sorghum, a strategy combining acidic ethanol extraction and ion precipitation was developed to extract highly active phenolic compounds from sweet sorghum fresh stalks, which increased the final total phenolic content to 276.79 mg gallic acid equivalents/g dried extract. The sweet sorghum phenolic compounds extract was rich in gallic, gentisic, trans-ferulic, caffeic, coumaric, chlorogenic, and p-hydroxybenzoic acids, showing strong antioxidant properties. Evaluation of cell membrane integrity and biomacromolecules (protein and nucleic acid) leakage revealed that the sweet sorghum phenolic compounds extract had significant bacteriostatic activity against Staphylococcus aureus CMCC26003 and Escherichia coli CMCC44102, suggesting potential antimicrobial effects. This study provided a novel strategy for comprehensive utilization of sweet sorghum.

来自能源作物甜高粱 [ Sorghum bicolor (L.) Moench ] 的生化生产引起了广泛关注。然而，尚未报道利用甜高粱生产酚类化合物。为提高甜高粱的综合利用率，采用酸性乙醇提取和离子沉淀相结合的策略，从甜高粱鲜茎中提取高活性酚类化合物，最终总酚含量提高到276.79 mg没食子酸当量/g干浸膏。甜高粱酚类化合物提取物富含没食子，龙胆酸，反式阿魏酸，咖啡酸，香豆酸，绿原，和p-羟基苯甲酸，显示出强大的抗氧化特性。对细胞膜完整性和生物大分子（蛋白质和核酸）泄漏的评估表明，甜高粱酚类化合物提取物对金黄色葡萄球菌CMCC26003 和大肠杆菌CMCC44102具有显着的抑菌活性，表明具有潜在的抗菌作用。本研究为甜高粱的综合利用提供了一种新策略。