In this work, a novel electrochemical aptasensor based on Fe-based MOFs was fabricated. To enhance sensitivity to the detection of antibiotics, NH₂-MIL-101(Fe)/CNF@AuNPs was synthesized by a combination of hydrothermal, electrospinning, pyrolysis and electrodeposition methods. Subsequently aptamer could be attached to the NH₂-MIL-101(Fe)/CNF@AuNPs through “Au-S” bonds. The electrical signals generated from the interaction between antibiotics and aptamers were amplified by the NH₂-MIL-101(Fe)/CNF@AuNPs aptasensor, resulting in a significant current/impedance response. Thus, signal generation and amplification were successfully realized. In addition, the Box-Behnken Design (BBD) strategy was adopted to optimize the detection conditions and greatly reduce experimental errors. Under optimized conditions, the tetracycline concentration detected by the sensor had a linear relationship with the impedance in the range of 0.1–10⁵ nM TC. The lowest detection limit was 0.01 nM. NH₂-MIL-101(Fe)/CNF electrospinning aptasensor exhibited good selectivity and stability. Furthermore, quantitative detection of tetracycline was achieved in real water samples. The sensor reported here could become a promising new strategy for detecting tetracycline in environmental water samples in the future.

在这项工作中，制备了一种基于铁基 MOF 的新型电化学适体传感器。为了提高对抗生素检测的灵敏度，NH ₂ -MIL-101(Fe)/CNF@AuNPs 通过水热、静电纺丝、热解和电沉积方法的组合合成。随后适体可以通过“Au-S”键连接到 NH ₂ -MIL-101(Fe)/CNF@AuNPs。抗生素和适体相互作用产生的电信号被 NH ₂放大-MIL-101(Fe)/CNF@AuNPs 适体传感器，导致显着的电流/阻抗响应。从而成功地实现了信号的产生和放大。此外，采用Box-Behnken Design（BBD）策略优化检测条件，大大减少实验误差。在优化条件下，传感器检测到的四环素浓度与阻抗在 0.1–10 ⁵ nM TC范围内呈线性关系。最低检测限为 0.01 nM。NH ₂-MIL-101(Fe)/CNF 静电纺丝适体传感器表现出良好的选择性和稳定性。此外，在实际水样中实现了四环素的定量检测。这里报道的传感器可能成为未来检测环境水样中四环素的一种有前途的新策略。