ABSTRACT

The objective of this study was to evaluate the effect of gaseous ozone on the physiological quality of corn seeds with different initial germination percentages. Seeds with initial germination percentages of 54.0% (low physiological quality) and 86.0% (high physiological quality) were used. Samples of 0.12 kg of seeds were exposed to different combinations of inlet ozone concentrations (10 and 30 mg L⁻¹) and exposure times (0, 30, 60, 90, and 120 min). A flow rate of 1.0 L min⁻¹ was used at a temperature of 25 °C. The percentage of germination, shoot, root, and seedling lengths, germination velocity index, and electrical conductivity were analyzed. Univariate and multivariate analysis were used for data analysis. Seeds with low initial germination percentage showed increase of 1.6 to 1.8 times in germination percentage and approximately 1.2 times in length of the seedlings when the inlet ozone concentration was 10 mg L⁻¹ and the exposure times were 30 and 120 min. Regarding seeds of high physiological quality, there was an increase in the germination velocity index of 1.1 to 1.2 times in ozonated seeds at 10 mg L⁻¹, compared to the non-ozonated seeds. According to the multivariate analysis, the inlet ozone concentration of 10 mg L⁻¹ associated with exposure times of 30 and 120 min showed similar results for seeds with low initial germination percentage. Based on the obtained results, we concluded that regardless the initial corn seeds quality, the recommended treatment is made by the combination of inlet ozone concentration of 10 mg L⁻¹ and exposure time of 30 min. Also, greater benefits are obtained from the application of ozone gas in corn seeds of low initial physiological quality. The ozonation proved to be a promising technique for seed treatment since it increased germination percentage and shoot and seedling lengths.

摘要

本研究的目的是评估气态臭氧对不同初始发芽率玉米种子生理品质的影响。使用初始发芽率 54.0%（低生理质量）和 86.0%（高生理质量）的种子。将 0.12 kg 种子样品暴露于入口臭氧浓度（10 和 30 mg L ^-1）和暴露时间（0、30、60、90 和 120 分钟）的不同组合中。1.0 L min ^{-1 的}流速在 25 °C 的温度下使用。分析了发芽率、芽、根和幼苗长度、发芽速度指数和电导率。单变量和多变量分析用于数据分析。当入口臭氧浓度为10 mg L ^-1和暴露时间为30和120 min时，初始发芽率低的种子发芽率增加1.6至1.8倍，幼苗长度增加约1.2倍。对于高生理品质的种子，与未臭氧化的种子相比，10mg L ^-1臭氧化的种子的发芽速度指数增加了1.1至1.2倍。根据多元分析，入口臭氧浓度为10 mg L ^-1与 30 和 120 分钟的暴露时间相关，对于具有低初始发芽率的种子显示出相似的结果。根据获得的结果，我们得出结论，无论最初的玉米种子质量如何，推荐的处理方法是结合 10 mg L ^-1的入口臭氧浓度和 30 分钟的暴露时间。此外，在初始生理质量低的玉米种子中应用臭氧气体可以获得更大的好处。臭氧化被证明是一种很有前途的种子处理技术，因为它增加了发芽率和枝条和幼苗的长度。