Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae), is widely used in augmentative biological control programs. However, there is no information on the behavior and biology of this parasitoid in stored tobacco. Thus, this study aims to evaluate the chemotactic response, parasitism, and mortality of females of T. pretiosum parasitizing eggs of Ephestia kuehniella (Zeller) (Lepidoptera: Pyralidae) in the presence of different tobacco types. The chemotactic responses of T. pretiosum females were observed with a double and multiple choice olfactometer. The odors of dry Burley and Virginia tobacco and of Virginia tobacco seedlings were evaluated. Treatments with or without the presence of E. kuehniella eggs were compared. To evaluate parasitism with and without choice, eggs of E. kuehniella adhered to the same tobacco used in the olfactometer. The eggs of E. kuehniella were offered to a female of T. pretiosum for 2 h, with adult parasitoid mortality recorded. In double-choice olfactometer there was no attraction of T. pretiosum females to the odors of the different types and classes of dry tobacco evaluated when compared to the control (air). Only tobacco seedlings and E. kuehniella eggs attracted the parasitoid. Females of T. pretiosum preferred tobacco infested with host eggs regardless of the type and class of tobacco. In multiple-choice olfactometer T. pretiosum females spent the same time in the area of the arms containing tobacco as in the area of arm containing control (air). The percentage of apparent parasitism of T. pretiosum varied according to the surface on which the host's eggs adhered. Although females of T. pretiosum responded positively to the host's volatiles, even with the presence of dry tobacco, when they came into direct contact with some types and classes of tobacco there was no parasitism.

Trichogramma pretiosum Riley（膜翅目：Trichogrammatidae），广泛用于增强生物控制程序。然而，没有关于这种寄生物在储存烟草中的行为和生物学的信息。因此，本研究旨在评估寄生于Ephestia kuehniella (Zeller) (Lepidoptera: Pyralidae )卵的T. pretiosum雌性在不同烟草类型存在下的趋化反应、寄生性和死亡率。用双选和多选嗅觉计观察T. pretiosum雌性的趋化反应。评估了干燥的白肋烟和弗吉尼亚烟草以及弗吉尼亚烟草幼苗的气味。存在或不存在E. kuehniella 的处理鸡蛋进行了比较。为了评估有无选择的寄生性，E 的卵。kuehniella粘附在嗅觉计中使用的相同烟草上。将E. kuehniella的卵提供给T. pretiosum的雌性2 小时，记录成虫的死亡率。在双选嗅觉计中，与对照（空气）相比，T. pretiosum雌性对所评估的不同类型和类别的干烟草的气味没有吸引力。只有烟草幼苗和E. kuehniella卵吸引了寄生蜂。无论烟草的类型和类别如何，T. pretiosum 的雌性都喜欢被寄主卵侵染的烟草。在多项选择嗅觉计T. pretiosum雌性在含有烟草的区域和含有控制（空气）的区域中的时间相同。T. pretiosum的明显寄生百分比根据寄主卵附着的表面而变化。尽管T. pretiosum 的雌性对宿主的挥发物反应积极，即使存在干烟草，当它们与某些类型和类别的烟草直接接触时，也没有寄生。