Sperm handling procedures and its usage for in vitro fertilization are crucial for standardized experimental operations on evaluation of reproductive performance, gamete quality, and optimization of fertilization protocols. In our study, the changes in perch sperm motility parameters within 6-h chilled storage and using 4 different activating solutions were compared. Eight different sperm-to-egg ratio was also compared during fertilization. Sperm activated with modified activating solution for cyprinids (78±11%), common perch activating solution (68±16%), modified Lahnsteiner activating solution (75±16%), and Woynárovich solution (76±13%) showed similar progressive motility at 10 s after activation. At 30 s after activation, progressive motility decreased below 5%, regardless the activating solution used. Progressive motility decreased significantly already after 2 h of storage (51±19%) in comparison with 0 h (78±5%). The highest average fertilization rate (using common perch activating solution) was observed with a sperm-to-egg ratio 2.5×10⁵:1 (80±9%), where the smallest variability in the values was also recorded (coefficient of variation: 11%). However, no significant difference was detected among the 8 sperm-to-egg ratio groups. According to our findings, undiluted fresh perch sperm is recommended to use in 1 h post-stripping. Modified Lahnsteiner’s activating solution can be applied efficiently for quality assessment where common perch activating solution is applicable for fertilization in Eurasian perch. A sperm-to-egg ratio 2.5×10⁵:1 already allows to achieve a high fertilization rate; however, the finding is needed to be tested also at hatchery level (higher number of eggs).

精子处理程序及其在体外受精中的使用对于评估生殖性能、配子质量和优化受精方案的标准化实验操作至关重要。在我们的研究中，比较了 6 小时冷藏和使用 4 种不同激活溶液的鲈鱼精子活力参数的变化。在受精过程中还比较了八种不同的精子与卵子的比例。用改良鲤科鱼活化液 (78±11%)、普通鲈鱼活化液 (68±16%)、改良 Lahnsteiner 活化液 (75±16%) 和 Woynárovich 液 (76±13%) 活化的精子表现出相似的进行性运动激活后 10 秒。激活后 30 秒，无论使用何种激活溶液，渐进性运动性都降低到 5% 以下。与 0 小时 (78±5%) 相比，储存 2 小时 (51±19%) 后进行性运动已经显着下降。平均受精率最高（使用普通鲈鱼活化液），精子与卵子的比例为 2.5×10⁵ :1 (80±9%)，其中还记录了值的最小变异性（变异系数：11%）。然而，在 8 个精卵比组之间没有检测到显着差异。根据我们的研究结果，建议在剥离后 1 小时内使用未稀释的新鲜鲈鱼精子。改良的 Lahnsteiner 活化液可有效应用于质量评估，其中普通鲈鱼活化液适用于欧亚鲈鱼的施肥。2.5×10 ⁵ :1的精卵比已经可以实现高受精率；然而，这一发现还需要在孵化场水平（更多的种蛋）进行测试。