One of the most important class of natural compounds with successful preclinical results in the management of cancer is the flavonoids. Due to the plethora of biological activities, apigenin (4,5,7 trihydroxyflavone) is a main representant of the flavone subclass. Although the antiproliferative and antiangiogenic effects of apigenin were studied on a significant number of human and murine melanoma cell lines, in order to complete the data existing in the literature, the aim of this study is to evaluate the in vitro effect of apigenin on SK-MEL-24 human melanoma cell line as well as in vivo on tumor angiogenesis using the aforementioned cell line on the chorioallantoic membrane assay. Results have shown that in the range of tested doses, the phytocompound presents significant antiproliferative, cytotoxic, and antimigratory potential at 30 μM, respectively, 60 μM. Moreover, the phytocompound in both tested concentrations limited melanoma cell growth and migration and induced a reduced angiogenic reaction limiting melanoma cell development.

中文翻译：

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黄酮芹菜素对 SK-MEL-24 人黑色素瘤细胞系的抗增殖和抗血管生成作用的体外体内评价

在癌症治疗中具有成功临床前结果的最重要的一类天然化合物是黄酮类化合物。由于过多的生物活性，芹菜素（4 , 5,7 三羟基黄酮）是黄酮亚类的主要代表。尽管在大量人和鼠黑色素瘤细胞系上研究了芹菜素的抗增殖和抗血管生成作用，但为了完善文献中存在的数据，本研究的目的是评估芹菜素对 SK-的体外作用。 MEL-24 人黑色素瘤细胞系以及体内在绒毛尿囊膜测定中使用上述细胞系对肿瘤血管生成进行研究。结果表明，在测试剂量范围内，植物化合物分别在 30 μM 和 60  μM时表现出显着的抗增殖、细胞毒性和抗迁移潜力 。此外，两种测试浓度的植物化合物都限制了黑色素瘤细胞的生长和迁移，并诱导了减少的血管生成反应限制了黑色素瘤细胞的发育。