Searching for new natural bioactive capping agents represent an urgent priority in the green synthesis of metal nanoparticles. Additionaly, the biosaftey of metal nanparticles is a major concern especially in medical applications. Recently, the use of pharmacollogicaly active natural products as capping agents has been deployed to avoid toxic effects during the nanoparticles preparation and to enhance their drugability compared with convential drugs. Helichrysum foetidum is a South African medicinal plant used in folk medicine for the treatment of different human pathologies, and it is known to contain a variety of bioactive compounds. Herein, the total extract and two pure chalcones, helichrysetin and helichrysin, isolated from the same plant were successfully used to synthesize quasi-monodispersed gold nanoparticles in the size range of 2–12 nm. The bio-evaluation of samples indicated that the AuNP/capping agent conjugates are biostable, and have different biological profiles from the total extract/pure compounds. The enzymatic inhibition assays showed significant inhibition by the total extract, helichrysetin and their gold nanoparticles. Interestingly, a similar activity was observed for glucose uptake in HEK293 treated cells. On the other hand, all the tested samples relatively demonstrated no cytotoxicity when tested against the HaCaT keratinocytes. In conclusion, the study demonstrated potential enhancement of glucose uptake in mammalian kidney cells, and inhibition of carbohydrate-hydrolysing enzymes by green synthesized gold nanoparticles of H. foetidum. It also provides a therapeutic appraisal of AuNPs/chalcones conjugate towards the development of antidiabetes drugs derived from H. foetidum and its gold nanoparticles.

寻找新的天然生物活性封端剂是金属纳米颗粒绿色合成的当务之急。此外，金属纳米颗粒的生物安全性是一个主要问题，尤其是在医疗应用中。最近，已部署使用药理活性天然产物作为封端剂，以避免纳米颗粒制备过程中的毒性作用，并与常规药物相比提高其成药性。蜡菊是一种南非药用植物，用于民间医学，用于治疗不同的人类疾病，众所周知，它含有多种生物活性化合物。在此，从同一植物中分离出的总提取物和两种纯查耳酮（蜡菊素和蜡菊素）成功地用于合成尺寸范围为 2-12 nm 的准单分散金纳米颗粒。样品的生物评估表明，AuNP/封端剂偶联物具有生物稳定性，并且与总提取物/纯化合物具有不同的生物学特征。酶促抑制试验显示总提取物、蜡菊素及其金纳米颗粒具有显着的抑制作用。有趣的是，在 HEK293 处理的细胞中观察到类似的葡萄糖摄取活性。另一方面，当针对 HaCaT 角质形成细胞进行测试时，所有测试样品都相对没有表现出细胞毒性。总之，该研究证明了哺乳动物肾细胞中葡萄糖摄取的潜在增强，以及绿色合成的金纳米粒子对碳水化合物水解酶的抑制作用。H. 恶臭。它还提供了 AuNPs/查尔酮缀合物的治疗评估，用于开发源自H. foetidum及其金纳米颗粒的抗糖尿病药物。