ABSTRACT

Introduction:Variations in blood pressure (BP) are, in part, genetically determined and some polymorphisms of renin-angiotensin- aldosterone system (RAAS) and synthase of endothelial nitric oxide (eNOS) have been related to hypertension (HT). Conversely, physical exercise is considered a non-pharmacological tool for HT control, treatment, and prevention.

Objective: The purpose of this study is to investigate the relationship between eNOS and RAAS polymorphisms, their epistatic interaction, and the respective humoral factors in the BP control in normotensive/pre-hypertension and hypertensive older adults and how this relationship can be modulated by training status (TS) level.

Methods:A total of 155 older adults (66.94 ± 6.83 years old) performed the following evaluations: AAHPERD battery test to determine the general functional fitness index (GFFI), systolic and diastolic blood pressure (SBP and DBP), blood collection for DNA extraction, analysis of eNOS gene polymorphisms rs2070744; rs61722009 and rs1799983 and RAAS polymorphisms rs699; rs1799752 and rs5186, and quantification of ACE activity (Fluorimetric Assay) and nitrite concentration (Chemiluminescence Method).

Results and Conclusion:Good TS level appears to exert greater influence on SBP for G2 and G3 (G1: 125.79 ± 14.03/ G2: 119.91 ± 11.72^/G3: 119.71 ± 10.85) and on NO₂ for G3 (G1: 0.42 ± 0.25/ G2: 0.54 ± 0.45/ G3: 0.71 ± 0.52). No associations were observed between eNOS and RAAS polymorphisms, but the epistasis was identified between eNOS polymorphism, rs2070744, and RAAS polymorphism, rs699, revealing a statistically significant interaction (p = .0235) with training score of 0.63, a training test accuracy of 0.61 and a cross-validation consistency of 10/10. This result suggests an increased risk of hypertension.

摘要

简介：血压 (BP) 的变化部分是由基因决定的，肾素-血管紧张素-醛固酮系统 (RAAS) 和内皮一氧化氮合酶 (eNOS) 的一些多态性与高血压 (HT) 相关。相反，体育锻炼被认为是控制、治疗和预防 HT 的非药物工具。

目的：本研究的目的是研究 eNOS 和 RAAS 多态性、它们的上位相互作用以及血压正常/高血压前期和高血压老年人血压控制中各自的体液因素之间的关系，以及如何通过训练调节这种关系状态 (TS) 级别。

方法：共有 155 名老年人（66.94±6.83 岁）进行了以下评估：AAHPERD 电池测试以确定一般功能健康指数（GFFI）、收缩压和舒张压（SBP 和 DBP）、采血用于 DNA 提取, eNOS基因多态性分析rs2070744；rs61722009 和 rs1799983 和 RAAS 多态性 rs699；rs1799752 和 rs5186，以及 ACE 活性（荧光分析）和亚硝酸盐浓度（化学发光法）的量化。

结果和结论：良好的 TS 水平似乎对 G2 和 G3 的 SBP（G1：125.79 ± 14.03/ G2：119.91 ± 11.72 ^/ G3：119.71 ± 10.85）和G3 的NO ₂产生更大影响（G1：0.25/±0） G2：0.54±0.45/G3：0.71±0.52）。在 eNOS 和 RAAS 多态性之间没有观察到关联，但在 eNOS 多态性 rs2070744 和 RAAS 多态性 rs699 之间确定了上位性，揭示了具有统计学意义的交互作用 ( p = .0235)，训练得分为 0.63，训练测试准确率为 0.61和 10/10 的交叉验证一致性。这一结果表明患高血压的风险增加。