The present study involved incorporation of two major QTLs for pre-harvest sprouting tolerance (PHST) in an Indian wheat cultivar named Lok1, which happens to be PHS susceptible. For transfer of two QTLs, two independent programmes with two different donors (AUS1408, CN19055) were utilized. The recipient cv. Lok1 was crossed with each of the two donors, followed by a number of backcrosses. Each backcross progeny was subjected to foreground and background selections. KASP assay was also used for confirming the presence of PHST QTL. In one case, PHST QTL was later also pyramided with a gene for high grain protein content (Gpc-B1) and a gene for leaf rust resistance (Lr24). The MAS derived lines were screened for PHS using simulated rain chambers leading to selection of 10 PHST lines. Four of these advanced lines carried all the three QTL/genes and exhibited high level of PHST (PHS score 2–3) associated with significant improvement in GPC and resistance against leaf rust.

本研究涉及在印度小麦品种 Lok1 中整合两个主要的收获前发芽耐受性 (PHST) QTL，该品种恰好对 PHS 敏感。为了转移两个 QTL，使用了两个不同供体（AUS1408、CN19055）的独立程序。收件人简历。Lok1 与两个供体中的每一个杂交，然后进行多次回交。对每个回交后代进行前景和背景选择。KASP 测定也用于确认 PHST QTL 的存在。在一个案例中，PHST QTL 后来还与高谷物蛋白含量基因 ( Gpc-B1 ) 和叶锈病抗性基因 ( Lr24 ) 金字塔化。使用模拟雨室对 MAS 衍生品系进行 PHS 筛选，最终选出 10 条 PHST 品系。其中四个先进品系携带全部三个 QTL/基因，并表现出高水平的 PHST（PHS 评分 2-3），与 GPC 和叶锈病抗性的显着改善相关。