Yangyin Tongnao (YYTN) prescription is used as a traditional Chinese herbal formula, and it has antioxidant activity that mainly contributes in the treatment of cardiovascular and cerebrovascular diseases. However, the compounds related to its antioxidant activity are still unknown. In the present study, the fingerprints of YYTN extracts under different extraction conditions were obtained by high performance liquid chromatography (HPLC) to identify the common peaks to all the samples processed. A 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay and ferric reducing antioxidant power (FRAP) assay were carried out to evaluate the antioxidant activity of the extracts. Spectrum-effect relationship between HPLC fingerprints and antioxidant activity of YYTN was assessed by Pearson product-moment correlation coefficient (PPMCC) and multiple linear regression analysis (MLRA). The results showed that peaks 5, 6, 13, 15, and 24 of the fingerprints were closely connected to antioxidant activity. Five peaks were identified: vanillic acid (P5), puerarin (P7), ferulic acid (P13), daidzein (P21), and formononetin (P23). Our study successfully established the spectrum-effect relationship between HPLC fingerprints and antioxidant activity of YYTN, which provided a general method for establishing quality standards with a combination of chromatography and antioxidant activity.

中文翻译：

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养阴通脑方HPLC指纹图谱与抗氧化活性的谱效关系

养阴通脑（YYTN）方为传统中药配方，具有抗氧化活性，主要有助于治疗心脑血管疾病。然而，与其抗氧化活性相关的化合物仍然未知。在本研究中，通过高效液相色谱（HPLC）获得了不同提取条件下YYTN提取物的指纹图谱，以识别所有处理样品的共同峰。采用 2,2-二苯基-1-三硝基苯肼 (DPPH) 自由基清除活性测定和铁还原抗氧化能力 (FRAP) 测定来评估提取物的抗氧化活性。通过 Pearson 乘积矩相关系数 (PPMCC) 和多元线性回归分析 (MLRA) 评估 HPLC 指纹图谱与 YYTN 抗氧化活性之间的谱效关系。结果表明，指纹图谱的5、6、13、15和24峰与抗氧化活性密切相关。鉴定出五个峰：香草酸 ( P5 )、葛根素 ( P7 )、阿魏酸 ( P13 )、大豆黄酮 ( P21 ) 和芒柄花素 ( P23 )。我们的研究成功建立了YYTN的HPLC指纹图谱与抗氧化活性之间的谱效关系，为建立色谱与抗氧化活性相结合的质量标准提供了通用方法。