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Light-inducible deformation of mitochondria in live cells
Cell Chemical Biology ( IF 8.6 ) Pub Date : 2021-06-21 , DOI: 10.1016/j.chembiol.2021.05.015
Yutong Song 1 , Peiyuan Huang 1 , Xiaoying Liu 1 , Zhihao Zhao 1 , Yijin Wang 1 , Bianxiao Cui 2 , Liting Duan 3
Affiliation  

Mitochondria, the powerhouse of the cell, are dynamic organelles that undergo constant morphological changes. Increasing evidence indicates that mitochondria morphologies and functions can be modulated by mechanical cues. However, the mechano-sensing and -responding properties of mitochondria and the relation between mitochondrial morphologies and functions are unclear due to the lack of methods to precisely exert mechano-stimulation on and deform mitochondria inside live cells. Here, we present an optogenetic approach that uses light to induce deformation of mitochondria by recruiting molecular motors to the outer mitochondrial membrane via light-activated protein-protein hetero-dimerization. Mechanical forces generated by motor proteins distort the outer membrane, during which the inner mitochondrial membrane can also be deformed. Moreover, this optical method can achieve subcellular spatial precision and be combined with different optical dimerizers and molecular motors. This method presents a mitochondria-specific mechano-stimulator for studying mitochondria mechanobiology and the interplay between mitochondria shapes and functions.



中文翻译:

活细胞中线粒体的光诱导变形

线粒体是细胞的动力源,是动态的细胞器,会经历不断的形态变化。越来越多的证据表明,线粒体的形态和功能可以通过机械信号进行调节。然而,由于缺乏精确地对活细胞内的线粒体施加机械刺激和变形的方法,线粒体的机械传感和响应特性以及线粒体形态和功能之间的关系尚不清楚。在这里,我们提出了一种光遗传学方法,该方法使用光通过光激活的蛋白质-蛋白质异二聚化将分子马达募集到线粒体外膜来诱导线粒体变形。运动蛋白产生的机械力使外膜变形,在此期间线粒体内膜也可能变形。而且,这种光学方法可以实现亚细胞空间精度,并与不同的光学二聚体和分子马达相结合。该方法提出了一种线粒体特异性机械刺激器,用于研究线粒体机械生物学以及线粒体形状和功能之间的相互作用。

更新日期:2021-06-21
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