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Translational autoregulation of RF2 protein inE. colithrough programmed frameshifting
Physical Review E ( IF 2.4 ) Pub Date : 2021-06-21 , DOI: 10.1103/physreve.103.062412
Ajeet K Sharma 1
Affiliation  

Various feedback mechanisms regulate the expression of different genes to ensure the required protein levels inside a cell. In this paper, we develop a kinetic model for one such mechanism that autoregulates RF2 protein synthesis in E. coli through programmed frameshifting. The model finds that the programmed frameshifting autoregulates RF2 protein synthesis by two independent mechanisms. First, it increases the rate of RF2 synthesis from each mRNA transcript at low RF2 concentration. Second, programmed frameshifting can dramatically increase the lifetime of RF2 transcripts when RF2 protein levels are lower than a threshold. This sharp increase in mRNA lifetime is caused by a first-order phase transition from a low to a high ribosome density on an RF2 transcript. The high ribosome density prevents the transcript's degradation by shielding it from nucleases, which increases its average lifetime and hence RF2 protein levels. Our study identifies this quality control mechanism that regulates the cellular protein levels by breaking the hierarchy of processes involved in gene expression.

中文翻译:

RF2蛋白在E中的翻译自动调节。大肠杆菌通过程序移码

各种反馈机制调节不同基因的表达,以确保细胞内所需的蛋白质水平。在本文中,我们为这样一种机制开发了一个动力学模型,该机制在大肠杆菌中自动调节 RF2 蛋白质合成通过程序化移码。该模型发现程序化移码通过两种独立的机制自动调节 RF2 蛋白质合成。首先,它在低 RF2 浓度下提高了每个 mRNA 转录物的 RF2 合成速率。其次,当 RF2 蛋白水平低于阈值时,程序化移码可以显着增加 RF2 转录本的寿命。mRNA 寿命的这种急剧增加是由 RF2 转录本上从低核糖体密度到高核糖体密度的一级相变引起的。高核糖体密度通过屏蔽核酸酶来防止转录物降解,从而增加其平均寿命,从而增加 RF2 蛋白质水平。
更新日期:2021-06-21
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